Efficiënte IDUA-genmutatiedetectie met gecombineerd gebruik van dHPLC en gedroogde bloedmonsters
Doelstellingen . Ontwikkeling van een eenvoudige, op mutatie gerichte methode om de kosten van mutatietesten te verlagen met behulp van een gemakkelijk verkrijgbaar biologisch materiaal. Beoordeling van de haalbaarheid van een dergelijke methode werd getest met behulp van een GC-rijk amplicon. Ontwerp en methoden . Een methode voor het denatureren van high-performance vloeistofchromatografie (dHPLC) werd verbeterd en geïmplementeerd als een techniek voor de detectie van varianten in exon 9 van het IDUA-gen . De geoptimaliseerde methode werd getest in 500 genomische DNA-monsters verkregen uit gedroogde bloedvlekken (DBS).
Resultaten. Met deze dHPLC-aanpak was het mogelijk om verschillende varianten te detecteren, waaronder de gemeenschappelijke p.Trp402Ter-mutatie in het IDUA-gen. Het hoge GC-gehalte interfereerde niet met de resolutie en betrouwbaarheid van deze techniek, en discriminatie van GC-transversies werd ook bereikt. Conclusie. Deze op PCR gebaseerde dHPLC-methode is een snelle , gevoelige en uitstekende optie gebleken voor het screenen van talrijke monsters verkregen uit DBS. Bovendien resulteerde het in de consistente detectie van duidelijk te onderscheiden profielen van de gemeenschappelijke p. Trp402Ter IDUA- mutatie met een voordelige balans tussen kosten en technische vereisten.
Vero-cel upstream bioprocesontwikkeling voor de productie van virale vectoren en vaccins
De Vero-cellijn wordt beschouwd als de meest gebruikte proceed cellijn voor de productie van virale vectoren en vaccins. Historisch gezien is het de eerste cellijn die door de WHO werd goedgekeurd voor de productie van menselijke vaccins. Uitgebreide experimentele gegevens over de productie van veel virussen met behulp van de Vero-cellijn zijn te vinden in de literatuur. De overgrote meerderheid van deze processen vertrouwt echter op de microcarrier-technologie. Hoewel dit systeem is opgezet voor de grootschalige productie van viraal vaccin, is het nog steeds vrij complicated en arbeidsintensief.
Bovendien blijft opschaling moeilijk en wordt deze beperkt door het door de dragers opgegeven oppervlak. Om deze en andere nadelen te overwinnen en efficiëntere productieprocessen tot stand te brengen, is het een prioriteit om het Vero-celplatform verder te ontwikkelen door nieuwe bioprocestechnologieën toe te passen. Vooral in tijden als de huidige COVID-19-pandemie kunnen geavanceerde en schaalbare platformtechnologieën efficiëntere en kosteneffectievere oplossingen bieden om aan de wereldwijde vraag naar vaccins te voldoen. Hierin bespreken we de heersende literatuur over de ontwikkeling van Vero-celbioprocessen voor de productie van virale vectoren en vaccins met als doel de recente vooruitgang in de ontwikkeling van bioprocessen te beoordelen.
We onderstrepen kritisch de noodzaak van verdere onderzoeksactiviteiten en beschrijven knelpunten om het Vero-celplatform te verbeteren door gebruik te maken van recente ontwikkelingen op het gebied van celcultuurtechnologie.

Efficiënte Inductie van cytotoxische T-cellen door Viral Vector Vaccinatie Vereist STING-Dependent DC Functies
Gemodificeerd vacciniavirus Ankara (MVA) is een verzwakte stam van het vacciniavirus en wordt momenteel onderzocht als een veelbelovende vaccinvector tegen infectieziekten en kanker. MVA verwierf mutaties in gastheerbereik en immunomodulerende genen, waardoor het virus deficiënt was voor replicatie in de meeste zoogdiercellen. MVA heeft een hoog veiligheidsprofiel en induceert robuuste immuunresponsen. De rol van aangeboren immuuntriggers voor de inductie van cytotoxische T-celreacties na vaccinatie is echter onvolledig begrepen.
Stimulator van interferon-genen (STING) is een adapter-eiwit dat de signalering stroomafwaarts van verschillende DNA-sensoren integreert en daardoor de inductie van sort I-interferonen en andere cytokinen of chemokinen bemiddelt als reactie op verschillende dsDNA-virussen. Omdat de sort I-interferonrespons volledig STING-afhankelijk was tijdens MVA-infectie, hebben we het impact van STING op primaire en secundaire cytotoxische T-celresponsen en geheugen T-celvorming na MVA-vaccinatie in STING KO-muizen bestudeerd .
Bovendien analyseerden we de impression van STING op de rijping van beenmerg-afgeleide dendritische cellen (BMDC’s) en hun functionaliteit als antigeenpresenterende cellen voor cytotoxische T-cellen tijdens MVA-infectie in vitro . Onze resultaten laten zien dat STING een impression heeft op de antigeenverwerking en presentatiecapaciteit van conventionele DC’s en een cruciale rol speelde voor DC-rijping en sort I interferonproductie. Belangrijk is dat STING nodig was voor de inductie van efficiënte cytotoxische T-celreacties in vivo , aangezien we important verminderde kortlevende effector- en effectorgeheugen-T-celreacties na priming in STING KO-muizen zagen.
Deze bevindingen geven aan dat STING waarschijnlijk aangeboren immuunsignalering stroomafwaarts van verschillende DNA-sensoren in DC’s integreert en de cytotoxische T-celrespons vormt by way of het DC-rijpingsfenotype dat sterk afhankelijk is van sort I interferonen in dit infectiemodel. Het begrijpen van de gedetailleerde functies van aangeboren immuuntriggers tijdens MVA-infectie zal bijdragen aan het geoptimaliseerde ontwerp van op MVA gebaseerde vaccins.
Selectie van chromatografische methoden voor de zuivering van uit celkweek afgeleid Orf- virus voor toepassing als vaccin of virale vector
In de afgelopen jaren is het Orf-virus een veelbelovend hulpmiddel geworden voor beschermende recombinante vaccins en oncolytische therapie. Geschikte methoden voor de productie van een Orf-virus , inclusief up- en downstream, zijn echter zeer beperkt. De gepresenteerde studie richt zich op downstream-verwerking en beschrijft de evaluatie van verschillende chromatografische eenheidsbewerkingen.
In deze context werden ionenuitwisselings-, pseudo-affiniteits- en sterische exclusiechromatografie toegepast voor de zuivering van het uit celkweek afgeleide Orf-virus, met als doel een maximale virusterugwinning en contaminantuitputting. De meest veelbelovende chromatografische methoden voor het vangen van de virusdeeltjes waren de sterische exclusie- of zouttolerante anionenuitwisselingsmembraanchromatografie, waarbij 84% en 86% van het infectieuze virus werd teruggewonnen.
Door de sterische exclusiechromatografie te combineren met een daaropvolgende Capto TM Core 700-hars- of hydrofobe interactiemembraanchromatografie als secundaire chromatografische stap, werd een totale virusterugwinning tot 76% bereikt. Bovendien was een volledige verwijdering van cellulair eiwit en een DNA-depletie van de gastheercel tot 82% mogelijk voor de sterische uitsluitingsmembranen en de Capto TM Core 700-combinatie. De studie onthult een reeks mogelijke eenheidsoperaties die geschikt zijn voor de chromatografische zuivering van het uit celkweek afgeleide Orf-virus, afhankelijk van de beoogde toepassing, dwz een humaan of veterinair gebruik, en de vereiste zuiverheid.
Anti-IL-8 Antibody |
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A2062-100 | Biovision | 100 µg | EUR 472.8 |
Anti-IL-8 Antibody |
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A00423 | BosterBio | 1 mL | EUR 1444.8 |
Description: Rabbit Polyclonal IL-8 Antibody. Validated in IP, WB and tested in Human. |
Anti-IL-8 antibody |
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STJ97710 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to IL-8. |
Anti-IL-8 antibody |
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STJ97717 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to IL-8. |
Anti-IL-8 antibody |
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STJ97720 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Mouse monoclonal to IL-8. |
Anti-IL-8 antibody |
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STJ98175 | St John's Laboratory | 100 µl | EUR 280.8 |
Description: Mouse monoclonal to IL-8. |
anti-IL-8 (3E4D8) |
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LF-MA30011 | Abfrontier | 100 ul | EUR 448.8 |
Description: Mouse Monoclonal to IL-8 |
mAb mouse anti-human IL-8 |
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CT264 | U-CyTech | 0.5 mg | EUR 312 |
IL-8/CXCL8, Human |
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HY-P7224 | MedChemExpress | 50ug | EUR 596.4 |
Human IL-8 Protein |
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abx060803-25ug | Abbexa | 25 ug | EUR 644.4 |
Human IL-8 Protein |
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abx060804-25ug | Abbexa | 25 ug | EUR 644.4 |
Recombinant Human IL-8 |
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P0180 | FN Test | 100ug | EUR 626.83 |
Description: Recombinant Human protein for IL-8 |
Recombinant Human IL-8 |
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SJB05-01 | Amyotop | 25µg/vial | EUR 342 |
Anti-IL-8 Monoclonal Antibody |
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A00423-1 | BosterBio | 100ul | EUR 476.4 |
Description: Mouse Monoclonal Antibody for IL-8 Antibody (CXCL8) detection. Tested with IHC in Human, Mouse, Rat. |
Human Interleukin-8 (IL-8) Antibody (Biotin Conjugate) |
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13102-05021 | AssayPro | 150 ug | EUR 313.2 |
Human IL-8 ELISA antibody pair |
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CT748-10 | U-CyTech | 10-plate | EUR 656.4 |
Human IL-8 ELISA antibody pair |
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CT748-20 | U-CyTech | 20-plate | EUR 1118.4 |
Human IL-8 Antibody (Biotin Conjugate) |
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33486-05121 | AssayPro | 150 ug | EUR 442.8 |
Human Interleukin 8 (IL-8) CLIA Kit |
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abx195916-96tests | Abbexa | 96 tests | EUR 990 |
Human Interleukin 8,IL-8 ELISA KIT |
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201-12-0090 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Interleukin 8, IL-8 ELISA KIT |
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CSB-E04641h-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid (CSF), tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Interleukin 8, IL-8 ELISA KIT |
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1-CSB-E04641h | Cusabio |
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Description: Quantitative sandwich ELISA kit for measuring Human Interleukin 8, IL-8 in samples from serum, cell culture supernates, saliva, urine, cerebrospinalfluid(CSF), tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human IL-8(Interleukin 8) ELISA Kit |
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EH0205 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
Human Interleukin 8(IL-8)ELISA Kit |
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GA-E0129HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human Interleukin 8(IL-8)ELISA Kit |
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GA-E0129HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human Interleukin 8(IL-8)ELISA Kit |
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QY-E04259 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Interleukin 8,IL-8 ELISA KIT |
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YLA1210HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Interleukin 8,IL-8 ELISA KIT |
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YLA1210HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Human Interleukin-8 (IL-8) ELISA Kit |
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LF-EK60006 | Abfrontier | 1×96T | EUR 948 |
human IL-8 His tag |
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E410A07-100 | EnoGene | 100μg | EUR 1152 |
Human IL-8 ELISA kit |
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CT212A | U-CyTech | 5-plate | EUR 554.4 |
Human IL-8 ELISA kit |
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LF-EK50165 | Abfrontier | 1×96T | EUR 777.6 |
IL-8 (Human) ELISA Kit |
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K4169-100 | Biovision | each | EUR 874.8 |
Human IL-8 ELISA Kit |
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EHI0063 | Abclonal | 96Tests | EUR 625.2 |
IL-8 ELISA KIT|Human |
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EF003990 | Lifescience Market | 96 Tests | EUR 826.8 |
IL-8/CXCL8, 72a.a., Human |
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HY-P7380 | MedChemExpress | 50ug | EUR 596.4 |
Human Interleukin-8 (IL-8) AssayLite Antibody (RPE Conjugate) |
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13102-05051 | AssayPro | 150 ug | EUR 513.6 |
Human Interleukin-8 (IL-8) AssayLite Antibody (APC Conjugate) |
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13102-05061 | AssayPro | 150 ug | EUR 513.6 |
Human Interleukin-8 (IL-8) AssayLite Antibody (FITC Conjugate) |
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13102-05041 | AssayPro | 150 ug | EUR 513.6 |
rec Monocyte IL-8 (human) |
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H-9625.0005 | Bachem | 5.0µg | EUR 194.4 |
Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8] |
rec Monocyte IL-8 (human) |
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H-9625.0025 | Bachem | 25.0µg | EUR 457.2 |
Description: Sum Formula: C372H600N106O106S4; CAS# [142298-00-8] |
Human Interleukin-8 (IL-8) AssayLite Antibody (PerCP Conjugate) |
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13102-05071 | AssayPro | 150 ug | EUR 565.2 |
rec Endothelial IL-8 (human) |
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H-3742.0010 | Bachem | 10.0µg | EUR 340.8 |
Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9] |
rec Endothelial IL-8 (human) |
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H-3742.0050 | Bachem | 50.0µg | EUR 1167.6 |
Description: Sum Formula: C397H644N114O111S4; CAS# [142298-01-9] |
Anti-bovine IL-8 (CXCL8) antibody |
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STJ15100008 | St John's Laboratory | 250 µg | EUR 403.2 |
Description: These monoclonal antibodies enable sensitive and specific detection of bovine IL-8 in ELISpot. |
Anti-bovine IL-8 (CXCL8) antibody |
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STJ15100009 | St John's Laboratory | 250 µg | EUR 403.2 |
Description: This monoclonal antibody enables sensitive and specific detection of bovine IL-8 in ELISA. |
Human IL-8 Recombinant Protein |
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R00423-8 | BosterBio | 5ug/vial | EUR 310.8 |
Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast. |
Recombinant Human IL-8 Protein |
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RP00052 | Abclonal | 5 μg | EUR 178.8 |
IL-8 Antibody |
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5149-100 | Biovision | each | EUR 405.6 |
IL-8 Antibody |
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BF0238 | Affbiotech | 200ul | EUR 540 |
IL-8 Antibody |
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F51235-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 140.25 |
Description: IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. |
IL-8 Antibody |
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F51235-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. |
IL-8 Antibody |
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R31932 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family. IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment. |
IL-8 Antibody |
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R30420 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: Interleukin-8(IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8(IL8) is a member of the CXC chemokine family(Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment. |
CLIA kit for Human IL-8 (Interleukin 8) |
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E-CL-H0048 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Human IL-8 (Interleukin 8) in samples from Serum, Plasma, Cell supernatant |
IL-8, CXCL8, Interleukin-8 (72 a.a.), human |
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RC212-19A | Bio Basic | 5ug | EUR 125.26 |
IL-8, CXCL8, Interleukin-8 (77 a.a.), human |
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RC212-19B | Bio Basic | 5ug | EUR 125.26 |
ELISA kit for Human IL-8 (Interleukin 8) |
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E-EL-H0048 | Elabscience Biotech | 1 plate of 96 wells | EUR 452.4 |
Description: A sandwich ELISA kit for quantitative measurement of Human IL-8 (Interleukin 8) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human Interleukin 8,IL-8 |
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EK0240 | SAB | 96 tests | EUR 723.6 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Interleukin 8,IL-8 in samples from serum, plasma, tissue homogenates and other biological fluids. |
Human Interleukin-8 (IL-8) AssayMax ELISA Kit |
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EI1008-1 | AssayPro | 96 Well Plate | EUR 572.4 |
Human IL-8 AssayLite Antibody (RPE Conjugate) |
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33486-05151 | AssayPro | 150 ug | EUR 513.6 |
Human IL-8 AssayLite Antibody (APC Conjugate) |
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33486-05161 | AssayPro | 150 ug | EUR 513.6 |
Human IL-8 AssayLite Antibody (FITC Conjugate) |
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70021-05141 | AssayPro | 150 ug | EUR 513.6 |
Human IL-8 AssayLite Antibody (FITC Conjugate) |
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33486-05141 | AssayPro | 150 ug | EUR 513.6 |
Recombinant Human Interleukin-8/IL-8 (C-6His) |
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CC97-10ug | Novoprotein | 10ug | EUR 175.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human Interleukin-8/IL-8 (C-6His) |
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CC97-1mg | Novoprotein | 1mg | EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human Interleukin-8/IL-8 (C-6His) |
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CC97-500ug | Novoprotein | 500ug | EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human Interleukin-8/IL-8 (C-6His) |
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CC97-50ug | Novoprotein | 50ug | EUR 406.8 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Human IL-8 AssayLite Antibody (PerCP Conjugate) |
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33486-05171 | AssayPro | 150 ug | EUR 565.2 |
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99) |
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C035-10ug | Novoprotein | 10ug | EUR 181.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99) |
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C035-1mg | Novoprotein | 1mg | EUR 2203.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99) |
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C035-500ug | Novoprotein | 500ug | EUR 1557.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ser28-Ser99) |
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C035-50ug | Novoprotein | 50ug | EUR 424.8 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99) |
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C037-10ug | Novoprotein | 10ug | EUR 181.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99) |
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C037-1mg | Novoprotein | 1mg | EUR 2203.2 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99) |
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C037-500ug | Novoprotein | 500ug | EUR 1557.6 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99) |
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C037-50ug | Novoprotein | 50ug | EUR 412.8 |
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
IL-8 |
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E5-00004 | EnoGene | 1mg | EUR 1152 |
ELISA kit for Human IL-8 |
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KET6018-48T | Abbkine | 48T | EUR 242.4 |
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human IL-8 |
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KET6018-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 1292.4 |
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human IL-8 |
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KET6018-96T | Abbkine | 96T | EUR 346.8 |
Description: Quantitative sandwich ELISA for measuring Human IL-8 in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Human IL-8 ELISA kit (4X96T) |
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LF-EK50166 | Abfrontier | 4×96T | EUR 2641.2 |
Human IL-8/CXCL8 ELISA Kit |
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RK00011 | Abclonal | 96 Tests | EUR 625.2 |
IL-8 (77 a.a.), human recombinant |
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4149-1000 | Biovision | each | EUR 4758 |
IL-8 (72 a.a.), human recombinant |
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4149M-1000 | Biovision | each | EUR 4758 |
IL-8 (72 a.a.), human recombinant |
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4149M-25 | Biovision | each | EUR 339.6 |
IL-8 (77 a.a.), human recombinant |
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4149-25 | Biovision | each | EUR 339.6 |
CymaxTM Human IL-8 ELISA Kit |
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LF-EK0262 | Abfrontier | 1×96T | EUR 477.6 |
IL-8 (77 a.a.), human recombinant |
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P1026-.025 | ApexBio | 25 µg | EUR 360 |
Description: Interleukin-8 (IL-8) is a proinflammatory CXC chemokine produced by macrophages, epithelial cells. IL-8 is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies |
IL-8 (77 a.a.), human recombinant |
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P1026-1 | ApexBio | 1 mg | EUR 5071.2 |
Description: Interleukin-8 (IL-8) is a proinflammatory CXC chemokine produced by macrophages, epithelial cells. IL-8 is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies |
Human IL-8 PicoKine ELISA Kit |
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EK0413 | BosterBio | 96 wells | EUR 510 |
Description: For quantitative detection of human IL-8 in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
IL-8 ELISA Kit (Human) (OKBB00192) |
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OKBB00192 | Aviva Systems Biology | 96 Tests | EUR 606 |
Description: Description of target: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 1 pg/ml |
IL-8/CXCL8 (CHO-expressed), Human |
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HY-P7379 | MedChemExpress | 50ug | EUR 596.4 |
Genorise® Recombinant human IL-8 |
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GR104177 | Genorise Scientific | 5 µg | EUR 285 |
Genorise® Biotinylated Anti-Human IL-8 Polyclonal Antibody |
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GR109009 | Genorise Scientific | 50 mg | EUR 398 |
IL-8 Polyclonal Antibody |
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ABP52884-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP52884-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP52884-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP51621-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP51621-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP51621-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP54886-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP54886-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
IL-8 Polyclonal Antibody |
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ABP54886-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of IL-8 from Human. This IL-8 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IL-8 |
Polyclonal IL-8 Antibody |
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APR00274G | Leading Biology | 0.1mg | EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IL-8 . This antibody is tested and proven to work in the following applications: |
IL-8 Polyclonal Antibody |
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41063-100ul | SAB | 100ul | EUR 302.4 |
IL-8 Polyclonal Antibody |
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41063-50ul | SAB | 50ul | EUR 224.4 |
IL-8 Polyclonal Antibody |
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ES5885-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for IHC, WB, ELISA |
IL-8 Polyclonal Antibody |
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ES5885-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for IHC, WB, ELISA |
IL-8 Polyclonal Antibody |
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ES2620-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
IL-8 Polyclonal Antibody |
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ES2620-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
IL-8 Polyclonal Antibody |
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ES3883-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
IL-8 Polyclonal Antibody |
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ES3883-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against IL-8 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
Nori® Human IL-8 ELISA Kit |
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GR106355 | Genorise Scientific | 96-well | EUR 461 |
Human IFN-γ / IL-8 FluoroSpot kit |
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CT858-F2 | U-CyTech | 2-plate | EUR 694.8 |
IL-8, Interleukin-8, monkey |
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RC222-19 | Bio Basic | 5ug | EUR 125.26 |
IL-8 Inhibitor |
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5-01378 | CHI Scientific | 4 x 5mg | Ask for price |
CXCL8/IL-8 |
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E21-C97 | EnoGene | 10ug | EUR 411.6 |
IL-8 Inhibitor |
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H-2268.0001 | Bachem | 1.0mg | EUR 200.4 |
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
IL-8 Inhibitor |
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H-2268.0005 | Bachem | 5.0mg | EUR 691.2 |
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
IL-8 Inhibitor |
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H-2268.0025 | Bachem | 25.0mg | EUR 2648.4 |
Description: Sum Formula: C45H66N18O7S; CAS# [138559-60-1] net |
CXCL8/IL-8 |
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GT15156 | Neuromics | 100 ug | EUR 631.2 |
Bovine(IL-8) |
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QY-E60063 | Qayee Biotechnology | 96T | EUR 511.2 |
Human Herpesvirus 8 Antibody (anti-HHV-8) ELISA Kit |
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abx055749-96tests | Abbexa | 96 tests | EUR 801.6 |
IL-8 Interleukin-8 (1-77 a.a) Human Recombinant Protein (CXCL8) |
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PROTP10145-1 | BosterBio | Regular: 25ug | EUR 380.4 |
Description: Interleukin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8904 Dalton. ;The IL-8 is purified by proprietary chromatographic techniques. |
Bovine Cytokines IL-8(Cytokines IL-8) ELISA Kit |
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QY-E60102 | Qayee Biotechnology | 96T | EUR 511.2 |
IL-8 (13F8) Monoclonal Antibody |
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EM1260-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Mouse Monoclonal antibody against IL-8 (13F8) from Human/ Rat/ Mouse. This antibody is tested and validated for IHC |
IL-8 (13F8) Monoclonal Antibody |
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EM1260-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Mouse Monoclonal antibody against IL-8 (13F8) from Human/ Rat/ Mouse. This antibody is tested and validated for IHC |
IL-8 ELISA Kit (Human) : 96 Wells (OKAG00043) |
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OKAG00043 | Aviva Systems Biology | 96 Wells | EUR 715.2 |
Description: Description of target: The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.;Species reactivity: Human;Application: ELISA;Assay info: Quantitative Colorimentric Sandwich ELISA;Sensitivity: 16 pg/mL |
Human IL-8 ELISA Kit, 96 tests, Quantitative |
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HIL-080-170 | Alpha Diagnostics | 1 kit | EUR 578.4 |
Recombinant Human IL-8 (72 a.a.) (CXCL8) Protein |
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PROTP10145-4 | BosterBio | 25ug | EUR 380.4 |
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues. |
Recombinant Human IL-8 (77 a.a.) (CXCL8) Protein |
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PROTP10145-5 | BosterBio | 25ug | EUR 380.4 |
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (endothelial-derived) is an 8.9 kDa protein containing 77 amino acid residues. |
IL-8 Polyclonal Conjugated Antibody |
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C41063 | SAB | 100ul | EUR 476.4 |
Genorise® Goat Anti-monkey IL-8 polyclonal antibody |
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GR105209 | Genorise Scientific | 100 ug | EUR 438 |
Genorise® Biotinylated Anti-sheep IL-8 Polyclonal Antibody |
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GR109106 | Genorise Scientific | 50 mg | EUR 398 |
Genorise® Biotinylated Anti-equine IL-8 Polyclonal Antibody |
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GR109030 | Genorise Scientific | 50 mg | EUR 398 |
Genorise® Biotinylated Anti-bovine IL-8 Polyclonal Antibody |
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GR109080 | Genorise Scientific | 50 mg | EUR 398 |
Genorise® Biotinylated Anti-canine IL-8 Polyclonal Antibody |
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GR109132 | Genorise Scientific | 50 mg | EUR 398 |
Genorise® Biotinylated Anti-porcine IL-8 Polyclonal Antibody |
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GR109005 | Genorise Scientific | 50 mg | EUR 398 |
Genorise® Biotinylated Anti-chicken IL-8 Polyclonal Antibody |
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GR109158 | Genorise Scientific | 50 mg | EUR 398 |
Horse Interleukin 8 (IL-8) ELISA |
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QY-E120050 | Qayee Biotechnology | 96T | EUR 573.6 |
IL-8, CXCL8, Interleukin-8, canine |
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RC242-19 | Bio Basic | 5ug | EUR 125.26 |
IL-8, CXCL8, Interleukin-8, porcine |
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RC282-19 | Bio Basic | 5ug | EUR 125.26 |
IL-8 Interleukin-8 (1-77 a.a.) Human Recombinant Protein, (CXCL8) Pichia |
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PROTP10145-3 | BosterBio | Regular: 25ug | EUR 380.4 |
Description: Interleukin-8 Human Recombinant produced in Yeast is a single, glycosylated polypeptide chain containing 79 amino acids and having a molecular mass of 9 kDa. ;The IL-8 is purified by proprietary chromatographic techniques. |
mAb rat anti-mouse/human IL-5 |
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CT694 | U-CyTech | 0.5 mg | EUR 312 |
Anti-IL-4Rα (Dupilumab), Human IgG4 Antibody |
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A2148-100 | Biovision | 100 µg | EUR 612 |
Anti-IL-1β (Canakinumab), Human IgG1 Antibody |
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A2144-100 | Biovision | 100 µg | EUR 564 |
Anti-IL-23α (Guselkumab), Human IgG1 Antibody |
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A2147-100 | Biovision | 100 µg | EUR 612 |
IL-8 Interleukin-8 (1-72 a.a.) HumanRecombinant Protein (CXCL8) |
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PROTP10145 | BosterBio | Regular: 25ug | EUR 380.4 |
Description: Interleukin-8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8452 Dalton. ;The IL-8 is purified by proprietary chromatographic techniques. |
Anti-IL-12β (Ustekinumab), Human IgG1 Antibody |
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A2137-100 | Biovision | 100 µg | EUR 612 |
Anti-IL-17α (Secukinumab), Human IgG1 Antibody |
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A2154-100 | Biovision | 100 µg | EUR 612 |
Anti-IL-17Rα (Brodalumab), Human IgG2 Antibody |
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A2155-100 | Biovision | 100 µg | EUR 612 |
Anti Human 17? HSD 8 Polyclonal Antibody |
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KR099 | Sceti | each | EUR 778.8 |
Description: The Anti Human 17? HSD 8 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur. |
IL-8 Recombinant Protein |
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90-492 | ProSci | 10 ug | EUR 405.6 |
Description: Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the alpha (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro. |
IL-8 Recombinant Protein |
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90-493 | ProSci | 50 ug | EUR 789.9 |
Description: Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the alpha (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro. |
IL-8 Recombinant Protein |
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91-026 | ProSci | 0.05 mg | EUR 651.3 |
Description: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states. |
IL-8 Recombinant Protein |
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91-028 | ProSci | 0.05 mg | EUR 619.8 |
Description: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states. |
IL-8 Recombinant Protein |
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40-331-0005mg | ProSci | 0.005 mg | EUR 311.1 |
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues. |
IL-8 Recombinant Protein |
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40-331-0025mg | ProSci | 0.025 mg | EUR 437.1 |
Description: IL-8 is a proinflammatory CXC chemokine that can signal through the CXCR1 and CXCR2 receptors. It is secreted by monocytes and endothelial cells. IL-8 chemoattracts and activates neutrophils. Recombinant human IL-8 (monocyte-derived) is an 8.4 kDa protein containing 72 amino acid residues. |
IL-8 Blocking Peptide |
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BF0238-BP | Affbiotech | 1mg | EUR 234 |