Antibody afhankelijke versterking en Sendai  Virus  als een mogelijke vaccin  Vector

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designblast

De uitdagingen van de ontwikkeling van vaccins tegen Betacoronaviruses: Antibody afhankelijke versterking en Sendai  Virus  als een mogelijke vaccin  Vector

 

Om een ​​effectief en veilig vaccin tegen bètacoronavirussen te ontwerpen, is het noodzakelijk om hun evolutionair conservatieve antigene determinanten te gebruiken die de combinatie van sterke humorale en celgemedieerde immuunresponsen zullen opwekken. Het richten op dergelijke determinanten minimaliseert het risico van antilichaamafhankelijke versterking van virale infectie. Dit fenomeen werd waargenomen in dierproeven met experimentele vaccins tegen SARS-CoV-1 en MERS-CoV die waren ontwikkeld op foundation van geïnactiveerd coronavirus of vectorconstructen die het spike-eiwit (S) van het virion tot expressie brengen.

De substitutie en glycosylering van bepaalde aminozuren in de antigene determinanten van het S-eiwit, evenals de conformationele veranderingen, kunnen tot hetzelfde impact leiden in een nieuw experimenteel vaccin tegen SARS-CoV-2. Het gebruik van meer conservatieve structurele en aanvullende virale eiwitten voor de antigene determinanten van het vaccin zal dit probleem helpen voorkomen. Deze evaluation schetst benaderingen voor het ontwikkelen van vaccins tegen het nieuwe SARS-CoV-2-coronavirus die zijn gebaseerd op niet-pathogene virale vectoren.

Voor een efficiënte preventie van infecties veroorzaakt door respiratoire pathogenen is het vermogen van het vaccin om mucosale immuniteit in de luchtwegen te stimuleren belangrijk. Een dergelijk vaccin kan worden ontwikkeld met behulp van een niet-pathogene Sendai-virusvector, omdat het intranasaal kan worden toegediend en een mucosale immuunrespons kan induceren die de antivirale barrière in de luchtwegen versterkt en een betrouwbare bescherming biedt tegen infectie.

Detectie van mutaties in gyrB met behulp van denaturerende hogedrukvloeistofchromatografie ( DHPLC ) tussen Salmonella enterica serovar Typhi en Paratyphi A.

 

Fluorochinolonresistentie wordt gemedieerd door mutaties in het chinolonenresistentiebepalende gebied (QRDR) van de topoisomerasegenen. Denaturerende hogedrukvloeistofchromatografie (DHPLC) werd geëvalueerd voor de detectie van klinisch belangrijke mutaties in gyrB bij Salmonella.

 

Salmonella Typhi en S. Paratyphi A, gekarakteriseerd voor mutatie in QRDR van gyrA, parC en parE, werden onderzocht op mutatie in gyrB door DHPLC en gevalideerd door sequencing.

 

De DHPLC-analyse was in staat om de testmutant te onderscheiden van isolaten met wildtype gyrB en mutanten te onderscheiden van andere mutanten op foundation van piekprofiel en verschuiving in retentietijd. Er werden drie sequentievarianten gedetecteerd op codon 464 en er werd ook een nieuwe mutatie Ser→Thr gedetecteerd . gyrB-mutatie was geassocieerd met niet-klassieke chinolonresistentie (NALS-CIPDS) in 34 isolaten van alleen S. Typhi en was verschillend van klassieke chinolonresistentie geassocieerd met gyrA-mutaties (NALR-CIPDS).

 

Opstelling van een protocol voor moleculaire diagnose van β-thalassemie-mutaties in Tunesië met behulp van denaturerende high-performance vloeistofchromatografie ( DHPLC ).

 

ACHTERGROND

β-thalassemie is een van de meest voorkomende autosomaal recessieve aandoeningen wereldwijd . Het vertoont een grote moleculaire heterogeniteit als gevolg van meer dan 200 oorzakelijke mutaties in het β-globine-gen. In Tunesië vertegenwoordigt β- thalassemie de meest voorkomende monogene hemoglobinestoornis met 2,21% van de dragers.

 

Efficiënte en betrouwbare screeningsmethoden voor mutaties zijn essentieel om geschikte preventieprogramma’s voor risicoparen op te zetten. Het doel van de huidige studie is om een ​​efficiënte methode te ontwikkelen op foundation van de denaturerende hogedrukvloeistofchromatografie (DHPLC) waarbij het hele β-globinegen (HBB) wordt gescreend op mutaties die ongeveer 90% van het spectrum beslaan.

 

METHODEN

We hebben de validatie uitgevoerd van een DHPLC-assay voor directe genotypering van 11 bekende β-thalassemie-mutaties in de Tunesische bevolking.

 

RESULTATEN

De DHPLC-assay werd vastgesteld op foundation van de analyse van 62 gearchiveerde β-thalassemiemonsters die eerder waren gegenotypeerd en vervolgens werden gevalideerd met volledige overeenstemming op 50 exams met blinde gerandomiseerde monsters die eerder waren gegenotypeerd met DNA-sequencing en met 96% consistentie op 40 monsters als een prospectieve studie.

CONCLUSIES

In vergelijking met andere genotyperingstechnieken kan de DHPLC-methode voldoen aan de vereisten van directe genotypering van bekende β-thalassemie-mutaties in Tunesië en kan het worden toegepast als een krachtig hulpmiddel voor de genetische screening van prenatale en postnatale individuen.

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anti-LIF

YF-PA12952 50 ug
EUR 435.6
Description: Mouse polyclonal to LIF

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-48T 48T
EUR 456
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-96T 96T
EUR 582
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-48Tests 48 Tests
EUR 696

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-96Tests 96 Tests
EUR 968.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-48Tests 48 Tests
EUR 668.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-96Tests 96 Tests
EUR 928.8

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-48Tests 48 Tests
EUR 459.6

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-96Tests 96 Tests
EUR 630

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-48Tests 48 Tests
EUR 696

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-96Tests 96 Tests
EUR 968.4

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-48Tests 48 Tests
EUR 640.8

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-96Tests 96 Tests
EUR 890.4

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-48Tests 48 Tests
EUR 666

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-96Tests 96 Tests
EUR 925.2

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-48Tests 48 Tests
EUR 639.6

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-96Tests 96 Tests
EUR 888

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-48Tests 48 Tests
EUR 440.4

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-96Tests 96 Tests
EUR 602.4

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-48Tests 48 Tests
EUR 666

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-96Tests 96 Tests
EUR 925.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-48Tests 48 Tests
EUR 613.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-96Tests 96 Tests
EUR 850.8

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

LIF, mouse

MO16102 100 ug
EUR 810

LIF, Mouse

HY-P7084 50ug
EUR 639.6

Anti-LIF Antibody

A01400 100ug/vial
EUR 352.8

anti- LIF antibody

LSMab09804 100 ug
EUR 463.2

anti- LIF antibody

FNab09804 100µg
EUR 658.5
Description: Antibody raised against LIF

Anti-LIF Antibody

PA1562 100ug/vial
EUR 352.8

Anti-LIF Antibody

PB9036 100ug/vial
EUR 400.8

Anti-LIF antibody

STJ96472 200 µl
EUR 236.4
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody

STJ29846 100 µl
EUR 332.4
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Anti-LIF antibody

STJ73631 100 µg
EUR 430.8

Anti-LIF (aa28-39) antibody

STJ72919 100 µg
EUR 430.8

Mouse LIF shRNA Plasmid

20-abx971321
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 625.2

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

LIF Antibody

6245-002mg 0.02 mg
EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

6245-01mg 0.1 mg
EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Peptide

6245P 0.05 mg
EUR 197.7
Description: (IN) LIF peptide

LIF antibody

70R-12299 100 ug
EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF Antibody

5216-100
EUR 379.2

LIF Antibody

5216-30T
EUR 175.2

LIF Peptide

43-200P 0.1 mg
EUR 405.6

LIF antibody

70R-14100 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody

70R-51295 100 ul
EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF protein

30R-2771 10 ug
EUR 442.8
Description: Purified recombinant Human LIF protein

LIF protein

30R-2772 10 ug
EUR 586.8
Description: Purified recombinant Mouse LIF protein

LIF Antibody

36950-100ul 100ul
EUR 302.4

LIF Antibody

25283-100ul 100ul
EUR 468

LIF siRNA

20-abx922546
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

LIF siRNA

20-abx922547
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

LIF antibody

70R-6230 50 ug
EUR 560.4
Description: Rabbit polyclonal LIF antibody

rHu LIF

AK9977-0005 5µg Ask for price

rHu LIF

AK9977-0025 25µg Ask for price

rHu LIF

AK9977-0100 100µg Ask for price

rHu LIF

AK9977-1000 1mg Ask for price

LIF Antibody

DF13730 100ul
EUR 420

LIF Antibody

1-CSB-PA198481
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

1-CSB-PA004761
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF, human

MO16101 100 ug
EUR 825.6

LIF antibody

PAab09804 100 ug
EUR 463.2

LIF, Human

HY-P7049 10ug
EUR 1920

LIF Antibody

R30639 100 ug
EUR 419

LIF Antibody

R31467 100 ug
EUR 419

LIF Antibody

R36251-100UG 100 ug
EUR 399

LIF Antibody

F49998-0.08ML 0.08 ml
EUR 165

LIF Antibody

F49998-0.4ML 0.4 ml
EUR 379

Human CellExp? LIF, mouse recombinant

7268-10
EUR 444

LIF ELISA Kit (Mouse) (OKBB00760)

OKBB00760 96 Wells
EUR 606
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

Mouse LIF PicoKine ELISA Kit

EK0580 96 wells
EUR 510
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

LIF ELISA Kit (Mouse) (OKEH04045)

OKEH04045 96 Wells
EUR 652.8
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

Lif ORF Vector (Mouse) (pORF)

ORF049145 1.0 ug DNA
EUR 607.2

Lif ORF Vector (Mouse) (pORF)

ORF049146 1.0 ug DNA
EUR 607.2

ELISA kit for Mouse LIF

EK5250 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

LIF, Mouse Recombinant , 1MIU/ml

L4500-001 1ml
EUR 406.8

LIF, Mouse Recombinant , 1MIU/ml

L4500-005 5x1ml
EUR 1431.6

LIF, Mouse Recombinant , 10MIU/ml

L4501-001 1ml
EUR 1744.8

Leukemia Inhibitory Factor (LIF), mouse

PR16102 5 ug
EUR 522

LIF Rabbit pAb

A1288-100ul 100 ul
EUR 369.6

LIF Rabbit pAb

A1288-200ul 200 ul
EUR 550.8

LIF Rabbit pAb

A1288-20ul 20 ul
EUR 219.6

LIF Rabbit pAb

A1288-50ul 50 ul
EUR 267.6

LIF, human recombinant

4923-10
EUR 366

LIF, human recombinant

4923-1000
EUR 6846

LIF, human recombinant

4923-50
EUR 796.8

LIF, murine recombinant

4924-10
EUR 444

LIF, murine recombinant

4924-1000
EUR 8203.2

LIF Polyclonal Antibody

41551-100ul 100ul
EUR 302.4

LIF Polyclonal Antibody

41551-50ul 50ul
EUR 224.4

LIF Recombinant Protein

40-682 5 ug
EUR 311.1
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.

LIF Recombinant Protein

40-684 5 ug
EUR 311.1
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds.

LIF Blocking Peptide

33R-4712 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of LIF antibody, catalog no. 70R-6230

Ontwikkeling van hoge resolutie DNA Melting Analyse voor gelijktijdige detectie van Potato Mop-Prime  Virus  en Zijn  Vector , Spongospora subterranea, in de bodem 

In deze studie werd een reeks duplex reverse transcriptie (RT)-PCR-gemedieerde hoge resolutie DNA-smelt (HRM)-analyses voor gelijktijdige detectie van aardappelmop-virus (PMTV) en zijn protistische vector, Spongospora subterranea f.sp. subterranea (Sss), ontwikkeld.

De aantasting van grond door PMTV werd gedetecteerd met behulp van een op tabak gebaseerd lokaassysteem . Totaal RNA geëxtraheerd uit de bodem leidde tot succesvolle RT-PCR-gelelektroforese-detectie van zowel PMTV als Sss. Om efficiëntere detectie te vergemakkelijken, werden nieuw ontworpen primerparen voor PMTV RNA-soorten (dwz RNA-Rep, -CP en -TGB) samen met de bestaande Sss-primers geanalyseerd met behulp van real-time RT-PCR.

De resulterende amplicons vertoonden smeltprofielen die gemakkelijk konden worden onderscheiden. Onder duplex RT-PCR-formaat leidden alle PMTV- en Sss-primercombinaties tot succesvolle detectie van respectieve PMTV-RNA-soorten en Sss in de monsters door DNA- smeltanalyses met hoge resolutie (HRM) . Toen de duplex HRM-assay werd toegepast op bodemmonsters die waren verzameld van zes velden op vier verschillende locaties in New Brunswick, Canada, werd een positieve detectie van PMTV en/of Sss gevonden in 63-100% monsters verzameld uit velden waarin met PMTV geïnfecteerde knollen waren waargenomen.

De monsters van velden waar noch PMTV- noch Sss-geïnfecteerde knollen waren waargenomen, resulteerden daarentegen in een negatieve detectie door de check. Aastabak bioassay voor PMTV en Sss leverde vergelijkbare resultaten op. Tussen 63% -83% en 100% van de grondmonsters die werden verzameld op PMTV-besmette velden leidden tot respectievelijk PMTV- en Sss-infecties in de lokaasplanten; terwijl er geen PMTV- of Sss-geïnfecteerde planten werden verkregen uit grondmonsters die waren verzameld op PMTV/Sss-vrije velden.

Versnelling van de morfogenetische cyclus van de  virale  vector  Aedes aegypti  larven voor snellere larvicide bioassays

 

Elke bioassay om nieuwe chemisch gesynthetiseerde larviciden of fytolarviciden te testen tegen Culicidae  en meer schadelijke muggensoorten, zoals  Aede s  aegypti  en  Aedes albopictus , die specifiek knokkelkoorts, gele koorts, chikungunya- viruskoorts  overbrengen, evenals het Zika-virus of  Anopheles gambiae , een vector voor malaria en philariasis, vereist duizenden goed ontwikkelde larven, bij voorkeur in het vierde stadium. De natuurlijke morfogenetische cyclus van  Aedes  spp., in het veld of in het laboratorium, kan bij kamertemperatuur (bijv. 25°C) tot 19 dagen duren vanaf het eerste permanente contact tussen levensvatbare eieren en wateren het laatste stadium van larvale groei of metamorfose tot vliegende volwassenen. Een versnelde sequentiële vervelling is dus wenselijk voor snellere bioassays van larviciden.

We hebben dit doel bereikt in  Aedes aegypti  met zeer beperkte strategische en goedkope toevoegingen aan voedsel, zoals kokoswater, melk of caseïne, gistextract en in mindere mate glycerol. Het van nature rijke kokoswater was uitstekend geschikt om snel de populatie van instar IV-larven te bereiken , de meest geavanceerde vóór de verpopping, wat ongeveer een week bespaart voor daaropvolgende larvicide bioassays. Verdunde melk, als een andere voedselbron, zorgde voor een nog snellere uiteindelijke vervelling en volwassenen zijn nuttig voor taxonomische doeleinden van muggen.

anti-LIF

YF-PA12952 50 ug
EUR 435.6
Description: Mouse polyclonal to LIF

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-p-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Ra-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-48T 48T
EUR 656.4
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-b-96T 96T
EUR 858
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-c-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-48T 48T
EUR 456
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

DLR-LIF-Hu-96T 96T
EUR 582
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-48Tests 48 Tests
EUR 696

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-b-96Tests 96 Tests
EUR 968.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-48Tests 48 Tests
EUR 668.4

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-c-96Tests 96 Tests
EUR 928.8

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-48Tests 48 Tests
EUR 459.6

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Hu-96Tests 96 Tests
EUR 630

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-48Tests 48 Tests
EUR 696

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-p-96Tests 96 Tests
EUR 968.4

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-48Tests 48 Tests
EUR 640.8

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RDR-LIF-Ra-96Tests 96 Tests
EUR 890.4

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-48Tests 48 Tests
EUR 666

Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-b-96Tests 96 Tests
EUR 925.2

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-48Tests 48 Tests
EUR 639.6

Canine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-c-96Tests 96 Tests
EUR 888

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-48Tests 48 Tests
EUR 440.4

Human Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Hu-96Tests 96 Tests
EUR 602.4

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-48Tests 48 Tests
EUR 666

Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-p-96Tests 96 Tests
EUR 925.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-48Tests 48 Tests
EUR 613.2

Rat Leukemia Inhibitory Factor (LIF) ELISA Kit

RD-LIF-Ra-96Tests 96 Tests
EUR 850.8

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

LIF, mouse

MO16102 100 ug
EUR 810

LIF, Mouse

HY-P7084 50ug
EUR 639.6

Anti-LIF Antibody

A01400 100ug/vial
EUR 352.8

anti- LIF antibody

LSMab09804 100 ug
EUR 463.2

anti- LIF antibody

FNab09804 100µg
EUR 658.5
Description: Antibody raised against LIF

Anti-LIF Antibody

PA1562 100ug/vial
EUR 352.8

Anti-LIF Antibody

PB9036 100ug/vial
EUR 400.8

Anti-LIF antibody

STJ96472 200 µl
EUR 236.4
Description: Rabbit polyclonal to LIF.

Anti-LIF antibody

STJ29846 100 µl
EUR 332.4
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Anti-LIF antibody

STJ73631 100 µg
EUR 430.8

Anti-LIF (aa28-39) antibody

STJ72919 100 µg
EUR 430.8

Mouse LIF shRNA Plasmid

20-abx971321
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse LIF ELISA Kit

EML0014 96Tests
EUR 625.2

Recombinant Mouse LIF Protein

R01400-1 100ug/vial
EUR 352.8

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

LIF Antibody

6245-002mg 0.02 mg
EUR 206.18
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Antibody

6245-01mg 0.1 mg
EUR 523.7
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.

LIF Peptide

6245P 0.05 mg
EUR 197.7
Description: (IN) LIF peptide

LIF antibody

70R-12299 100 ug
EUR 483.6
Description: Rabbit polyclonal LIF antibody

LIF Antibody

5216-100
EUR 379.2

LIF Antibody

5216-30T
EUR 175.2

LIF Peptide

43-200P 0.1 mg
EUR 405.6

LIF antibody

70R-14100 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal LIF antibody

LIF antibody

70R-51295 100 ul
EUR 292.8
Description: Purified Polyclonal LIF antibody

LIF protein

30R-2771 10 ug
EUR 442.8
Description: Purified recombinant Human LIF protein

LIF protein

30R-2772 10 ug
EUR 586.8
Description: Purified recombinant Mouse LIF protein

LIF Antibody

36950-100ul 100ul
EUR 302.4

LIF Antibody

25283-100ul 100ul
EUR 468

LIF siRNA

20-abx922546
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

LIF siRNA

20-abx922547
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

LIF antibody

70R-6230 50 ug
EUR 560.4
Description: Rabbit polyclonal LIF antibody

rHu LIF

AK9977-0005 5µg Ask for price

rHu LIF

AK9977-0025 25µg Ask for price

rHu LIF

AK9977-0100 100µg Ask for price

rHu LIF

AK9977-1000 1mg Ask for price

LIF Antibody

DF13730 100ul
EUR 420

LIF Antibody

1-CSB-PA198481
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100

LIF Antibody

1-CSB-PA004761
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

LIF Antibody

1-CSB-PA012928ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA

LIF, human

MO16101 100 ug
EUR 825.6

LIF antibody

PAab09804 100 ug
EUR 463.2

LIF, Human

HY-P7049 10ug
EUR 1920

LIF Antibody

R30639 100 ug
EUR 419

LIF Antibody

R31467 100 ug
EUR 419

LIF Antibody

R36251-100UG 100 ug
EUR 399

LIF Antibody

F49998-0.08ML 0.08 ml
EUR 165

LIF Antibody

F49998-0.4ML 0.4 ml
EUR 379

Human CellExp? LIF, mouse recombinant

7268-10
EUR 444

LIF ELISA Kit (Mouse) (OKBB00760)

OKBB00760 96 Wells
EUR 606
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

Mouse LIF PicoKine ELISA Kit

EK0580 96 wells
EUR 510
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

LIF ELISA Kit (Mouse) (OKEH04045)

OKEH04045 96 Wells
EUR 652.8
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL

Lif ORF Vector (Mouse) (pORF)

ORF049145 1.0 ug DNA
EUR 607.2

Lif ORF Vector (Mouse) (pORF)

ORF049146 1.0 ug DNA
EUR 607.2

ELISA kit for Mouse LIF

EK5250 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.

LIF, Mouse Recombinant , 1MIU/ml

L4500-001 1ml
EUR 406.8

LIF, Mouse Recombinant , 1MIU/ml

L4500-005 5x1ml
EUR 1431.6

LIF, Mouse Recombinant , 10MIU/ml

L4501-001 1ml
EUR 1744.8

Leukemia Inhibitory Factor (LIF), mouse

PR16102 5 ug
EUR 522

LIF Rabbit pAb

A1288-100ul 100 ul
EUR 369.6

LIF Rabbit pAb

A1288-200ul 200 ul
EUR 550.8

LIF Rabbit pAb

A1288-20ul 20 ul
EUR 219.6

LIF Rabbit pAb

A1288-50ul 50 ul
EUR 267.6

LIF, human recombinant

4923-10
EUR 366

LIF, human recombinant

4923-1000
EUR 6846

LIF, human recombinant

4923-50
EUR 796.8

LIF, murine recombinant

4924-10
EUR 444

LIF, murine recombinant

4924-1000
EUR 8203.2

LIF Polyclonal Antibody

41551-100ul 100ul
EUR 302.4

LIF Polyclonal Antibody

41551-50ul 50ul
EUR 224.4

LIF Recombinant Protein

40-682 5 ug
EUR 311.1
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.

LIF Recombinant Protein

40-684 5 ug
EUR 311.1
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds.

LIF Blocking Peptide

33R-4712 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of LIF antibody, catalog no. 70R-6230

 

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