Antibody afhankelijke versterking en Sendai  Virus  als een mogelijke vaccin  Vector

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De uitdagingen van de ontwikkeling van vaccins tegen Betacoronaviruses: Antibody afhankelijke versterking en Sendai  Virus  als een mogelijke vaccin  Vector

 

Om een ​​effectief en veilig vaccin tegen bètacoronavirussen te ontwerpen, is het noodzakelijk om hun evolutionair conservatieve antigene determinanten te gebruiken die de combinatie van sterke humorale en celgemedieerde immuunresponsen zullen opwekken. Het richten op dergelijke determinanten minimaliseert het risico van antilichaamafhankelijke versterking van virale infectie. Dit fenomeen werd waargenomen in dierproeven met experimentele vaccins tegen SARS-CoV-1 en MERS-CoV die waren ontwikkeld op foundation van geïnactiveerd coronavirus of vectorconstructen die het spike-eiwit (S) van het virion tot expressie brengen.

De substitutie en glycosylering van bepaalde aminozuren in de antigene determinanten van het S-eiwit, evenals de conformationele veranderingen, kunnen tot hetzelfde impact leiden in een nieuw experimenteel vaccin tegen SARS-CoV-2. Het gebruik van meer conservatieve structurele en aanvullende virale eiwitten voor de antigene determinanten van het vaccin zal dit probleem helpen voorkomen. Deze evaluation schetst benaderingen voor het ontwikkelen van vaccins tegen het nieuwe SARS-CoV-2-coronavirus die zijn gebaseerd op niet-pathogene virale vectoren.

Voor een efficiënte preventie van infecties veroorzaakt door respiratoire pathogenen is het vermogen van het vaccin om mucosale immuniteit in de luchtwegen te stimuleren belangrijk. Een dergelijk vaccin kan worden ontwikkeld met behulp van een niet-pathogene Sendai-virusvector, omdat het intranasaal kan worden toegediend en een mucosale immuunrespons kan induceren die de antivirale barrière in de luchtwegen versterkt en een betrouwbare bescherming biedt tegen infectie.

Detectie van mutaties in gyrB met behulp van denaturerende hogedrukvloeistofchromatografie ( DHPLC ) tussen Salmonella enterica serovar Typhi en Paratyphi A.

 

Fluorochinolonresistentie wordt gemedieerd door mutaties in het chinolonenresistentiebepalende gebied (QRDR) van de topoisomerasegenen. Denaturerende hogedrukvloeistofchromatografie (DHPLC) werd geëvalueerd voor de detectie van klinisch belangrijke mutaties in gyrB bij Salmonella.

 

Salmonella Typhi en S. Paratyphi A, gekarakteriseerd voor mutatie in QRDR van gyrA, parC en parE, werden onderzocht op mutatie in gyrB door DHPLC en gevalideerd door sequencing.

 

De DHPLC-analyse was in staat om de testmutant te onderscheiden van isolaten met wildtype gyrB en mutanten te onderscheiden van andere mutanten op foundation van piekprofiel en verschuiving in retentietijd. Er werden drie sequentievarianten gedetecteerd op codon 464 en er werd ook een nieuwe mutatie Ser→Thr gedetecteerd . gyrB-mutatie was geassocieerd met niet-klassieke chinolonresistentie (NALS-CIPDS) in 34 isolaten van alleen S. Typhi en was verschillend van klassieke chinolonresistentie geassocieerd met gyrA-mutaties (NALR-CIPDS).

 

Opstelling van een protocol voor moleculaire diagnose van β-thalassemie-mutaties in Tunesië met behulp van denaturerende high-performance vloeistofchromatografie ( DHPLC ).

 

ACHTERGROND

β-thalassemie is een van de meest voorkomende autosomaal recessieve aandoeningen wereldwijd . Het vertoont een grote moleculaire heterogeniteit als gevolg van meer dan 200 oorzakelijke mutaties in het β-globine-gen. In Tunesië vertegenwoordigt β- thalassemie de meest voorkomende monogene hemoglobinestoornis met 2,21% van de dragers.

 

Efficiënte en betrouwbare screeningsmethoden voor mutaties zijn essentieel om geschikte preventieprogramma’s voor risicoparen op te zetten. Het doel van de huidige studie is om een ​​efficiënte methode te ontwikkelen op foundation van de denaturerende hogedrukvloeistofchromatografie (DHPLC) waarbij het hele β-globinegen (HBB) wordt gescreend op mutaties die ongeveer 90% van het spectrum beslaan.

 

METHODEN

We hebben de validatie uitgevoerd van een DHPLC-assay voor directe genotypering van 11 bekende β-thalassemie-mutaties in de Tunesische bevolking.

 

RESULTATEN

De DHPLC-assay werd vastgesteld op foundation van de analyse van 62 gearchiveerde β-thalassemiemonsters die eerder waren gegenotypeerd en vervolgens werden gevalideerd met volledige overeenstemming op 50 exams met blinde gerandomiseerde monsters die eerder waren gegenotypeerd met DNA-sequencing en met 96% consistentie op 40 monsters als een prospectieve studie.

CONCLUSIES

In vergelijking met andere genotyperingstechnieken kan de DHPLC-methode voldoen aan de vereisten van directe genotypering van bekende β-thalassemie-mutaties in Tunesië en kan het worden toegepast als een krachtig hulpmiddel voor de genetische screening van prenatale en postnatale individuen.

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Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
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Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-48Tests 48 Tests
EUR 511
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-96Tests 96 Tests
EUR 709
anti-LIF
YF-PA12952 50 ug
EUR 363
Description: Mouse polyclonal to LIF
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-48T 48T
EUR 547
  • Should the Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-96T 96T
EUR 715
  • Should the Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-48T 48T
EUR 527
  • Should the Canine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-96T 96T
EUR 688
  • Should the Canine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-48T 48T
EUR 380
  • Should the Human Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-96T 96T
EUR 485
  • Should the Human Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-48T 48T
EUR 547
  • Should the Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-96T 96T
EUR 715
  • Should the Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-48T 48T
EUR 508
  • Should the Rat Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-96T 96T
EUR 661
  • Should the Rat Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-48Tests 48 Tests
EUR 555
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-96Tests 96 Tests
EUR 771
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-48Tests 48 Tests
EUR 533
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-96Tests 96 Tests
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Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-48Tests 48 Tests
EUR 367
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-96Tests 96 Tests
EUR 502
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-48Tests 48 Tests
EUR 555
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-96Tests 96 Tests
EUR 771
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-48Tests 48 Tests
EUR 511
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-96Tests 96 Tests
EUR 709
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-48Tests 48 Tests
EUR 580
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-96Tests 96 Tests
EUR 807
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-48Tests 48 Tests
EUR 557
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-96Tests 96 Tests
EUR 774
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-48Tests 48 Tests
EUR 383
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-96Tests 96 Tests
EUR 525
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-48Tests 48 Tests
EUR 580
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-96Tests 96 Tests
EUR 807
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-48Tests 48 Tests
EUR 534
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-96Tests 96 Tests
EUR 742
anti- LIF antibody
FNab09804 100µg
EUR 548.75
  • Recommended dilution: WB: 1:200-1:1000
  • IHC: 1:50-1:500
  • Immunogen: leukemia inhibitory factor
  • Uniprot ID: P15018
  • Research Area: Signal Transduction, Immunology, Developmental biology, Neuroscience, Stem Cells
Description: Antibody raised against LIF
Anti-LIF Antibody
A01400 100ug/vial
EUR 294
Anti-LIF Antibody
PB9036 100ug/vial
EUR 334
anti- LIF antibody
LSMab09804 100 ug
EUR 386
Anti-LIF Antibody
PA1562 100ug/vial
EUR 294
Anti-LIF antibody
STJ73631 100 µg
EUR 359
Anti-LIF antibody
STJ96472 200 µl
EUR 197
Description: Rabbit polyclonal to LIF.
Anti-LIF antibody
STJ29846 100 µl
EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
LIF, Mouse
HY-P7084 50ug
EUR 533
LIF, mouse
MO16102 100 ug
EUR 675
Anti-LIF (aa28-39) antibody
STJ72919 100 µg
EUR 359
Mouse LIF ELISA Kit
EML0014 96Tests
EUR 521
Mouse LIF shRNA Plasmid
20-abx971321
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
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Recombinant Mouse LIF Protein
R01400-1 100ug/vial
EUR 294
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
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rHu LIF
AK9977-0005 5µg Ask for price
rHu LIF
AK9977-0025 25µg Ask for price
rHu LIF
AK9977-0100 100µg Ask for price
rHu LIF
AK9977-1000 1mg Ask for price
LIF, Human
HY-P7049 10ug
EUR 1600
LIF siRNA
20-abx922546
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  • EUR 732.00
  • 15 nmol
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LIF siRNA
20-abx922547
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  • 15 nmol
  • 30 nmol
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LIF antibody
70R-51295 100 ul
EUR 244
Description: Purified Polyclonal LIF antibody
LIF antibody
70R-6230 50 ug
EUR 467
Description: Rabbit polyclonal LIF antibody
LIF Antibody
36950-100ul 100ul
EUR 252
LIF Antibody
5216-100
EUR 316
LIF Antibody
5216-30T
EUR 146
LIF protein
30R-2771 10 ug
EUR 369
Description: Purified recombinant Human LIF protein
LIF protein
30R-2772 10 ug
EUR 489
Description: Purified recombinant Mouse LIF protein
LIF Antibody
25283-100ul 100ul
EUR 390
LIF antibody
70R-12299 100 ug
EUR 403
Description: Rabbit polyclonal LIF antibody
LIF antibody
70R-14100 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal LIF antibody
LIF Antibody
1-CSB-PA004761
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
LIF Antibody
1-CSB-PA012928ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Antigen Affinity Purified
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA
LIF Antibody
1-CSB-PA198481
  • EUR 317.00
  • EUR 244.00
  • 100ul
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  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100
LIF antibody
PAab09804 100 ug
EUR 386
LIF, human
MO16101 100 ug
EUR 688
ELISA kit for Mouse LIF
EK5250 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.
Mouse LIF PicoKine ELISA Kit
EK0580 96 wells
EUR 425
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).
Human CellExp? LIF, mouse recombinant
7268-10
EUR 370
Leukemia Inhibitory Factor (LIF), mouse
PR16102 5 ug
EUR 435
LIF, Mouse Recombinant , 1MIU/ml
L4500-001 1ml
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LIF, Mouse Recombinant , 1MIU/ml
L4500-005 5x1ml
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LIF, Mouse Recombinant , 10MIU/ml
L4501-001 1ml
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Lif ORF Vector (Mouse) (pORF)
ORF049145 1.0 ug DNA
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Lif ORF Vector (Mouse) (pORF)
ORF049146 1.0 ug DNA
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LIF ELISA Kit (Mouse) (OKEH04045)
OKEH04045 96 Wells
EUR 544
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL
LIF ELISA Kit (Mouse) (OKBB00760)
OKBB00760 96 Wells
EUR 505
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml
Polyclonal LIF Antibody
APR00279G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LIF . This antibody is tested and proven to work in the following applications:
LIF Conjugated Antibody
C36950 100ul
EUR 397
Polyclonal LIF Antibody
APR06696G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LIF . This antibody is tested and proven to work in the following applications:
LIF Polyclonal Antibody
ES3837-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
LIF Polyclonal Antibody
ES3837-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
LIF Blocking Peptide
20-abx064170
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
LIF Polyclonal Antibody
ABP52838-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Polyclonal Antibody
ABP52838-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Polyclonal Antibody
ABP52838-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Rabbit pAb
A1288-100ul 100 ul
EUR 308
LIF Rabbit pAb
A1288-200ul 200 ul
EUR 459
LIF Rabbit pAb
A1288-20ul 20 ul
EUR 183
LIF Rabbit pAb
A1288-50ul 50 ul
EUR 223
LIF Blocking Peptide
33R-4712 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of LIF antibody, catalog no. 70R-6230
LIF Polyclonal Antibody
41551-100ul 100ul
EUR 252
LIF Polyclonal Antibody
41551-50ul 50ul
EUR 187
LIF, human recombinant
4923-10
EUR 305
LIF, human recombinant
4923-1000
EUR 5705
LIF, human recombinant
4923-50
EUR 664
LIF, murine recombinant
4924-10
EUR 370
LIF, murine recombinant
4924-1000
EUR 6836

Ontwikkeling van hoge resolutie DNA Melting Analyse voor gelijktijdige detectie van Potato Mop-Prime  Virus  en Zijn  Vector , Spongospora subterranea, in de bodem 

In deze studie werd een reeks duplex reverse transcriptie (RT)-PCR-gemedieerde hoge resolutie DNA-smelt (HRM)-analyses voor gelijktijdige detectie van aardappelmop-virus (PMTV) en zijn protistische vector, Spongospora subterranea f.sp. subterranea (Sss), ontwikkeld.

De aantasting van grond door PMTV werd gedetecteerd met behulp van een op tabak gebaseerd lokaassysteem . Totaal RNA geëxtraheerd uit de bodem leidde tot succesvolle RT-PCR-gelelektroforese-detectie van zowel PMTV als Sss. Om efficiëntere detectie te vergemakkelijken, werden nieuw ontworpen primerparen voor PMTV RNA-soorten (dwz RNA-Rep, -CP en -TGB) samen met de bestaande Sss-primers geanalyseerd met behulp van real-time RT-PCR.

De resulterende amplicons vertoonden smeltprofielen die gemakkelijk konden worden onderscheiden. Onder duplex RT-PCR-formaat leidden alle PMTV- en Sss-primercombinaties tot succesvolle detectie van respectieve PMTV-RNA-soorten en Sss in de monsters door DNA- smeltanalyses met hoge resolutie (HRM) . Toen de duplex HRM-assay werd toegepast op bodemmonsters die waren verzameld van zes velden op vier verschillende locaties in New Brunswick, Canada, werd een positieve detectie van PMTV en/of Sss gevonden in 63-100% monsters verzameld uit velden waarin met PMTV geïnfecteerde knollen waren waargenomen.

De monsters van velden waar noch PMTV- noch Sss-geïnfecteerde knollen waren waargenomen, resulteerden daarentegen in een negatieve detectie door de check. Aastabak bioassay voor PMTV en Sss leverde vergelijkbare resultaten op. Tussen 63% -83% en 100% van de grondmonsters die werden verzameld op PMTV-besmette velden leidden tot respectievelijk PMTV- en Sss-infecties in de lokaasplanten; terwijl er geen PMTV- of Sss-geïnfecteerde planten werden verkregen uit grondmonsters die waren verzameld op PMTV/Sss-vrije velden.

Versnelling van de morfogenetische cyclus van de  virale  vector  Aedes aegypti  larven voor snellere larvicide bioassays

 

Elke bioassay om nieuwe chemisch gesynthetiseerde larviciden of fytolarviciden te testen tegen Culicidae  en meer schadelijke muggensoorten, zoals  Aede s  aegypti  en  Aedes albopictus , die specifiek knokkelkoorts, gele koorts, chikungunya- viruskoorts  overbrengen, evenals het Zika-virus of  Anopheles gambiae , een vector voor malaria en philariasis, vereist duizenden goed ontwikkelde larven, bij voorkeur in het vierde stadium. De natuurlijke morfogenetische cyclus van  Aedes  spp., in het veld of in het laboratorium, kan bij kamertemperatuur (bijv. 25°C) tot 19 dagen duren vanaf het eerste permanente contact tussen levensvatbare eieren en wateren het laatste stadium van larvale groei of metamorfose tot vliegende volwassenen. Een versnelde sequentiële vervelling is dus wenselijk voor snellere bioassays van larviciden.

We hebben dit doel bereikt in  Aedes aegypti  met zeer beperkte strategische en goedkope toevoegingen aan voedsel, zoals kokoswater, melk of caseïne, gistextract en in mindere mate glycerol. Het van nature rijke kokoswater was uitstekend geschikt om snel de populatie van instar IV-larven te bereiken , de meest geavanceerde vóór de verpopping, wat ongeveer een week bespaart voor daaropvolgende larvicide bioassays. Verdunde melk, als een andere voedselbron, zorgde voor een nog snellere uiteindelijke vervelling en volwassenen zijn nuttig voor taxonomische doeleinden van muggen.

Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Mu-96Tests 96 Tests
EUR 677
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-48Tests 48 Tests
EUR 511
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Mu-96Tests 96 Tests
EUR 709
anti-LIF
YF-PA12952 50 ug
EUR 363
Description: Mouse polyclonal to LIF
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-48T 48T
EUR 547
  • Should the Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-b-96T 96T
EUR 715
  • Should the Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-48T 48T
EUR 527
  • Should the Canine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-c-96T 96T
EUR 688
  • Should the Canine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-48T 48T
EUR 380
  • Should the Human Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Hu-96T 96T
EUR 485
  • Should the Human Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-48T 48T
EUR 547
  • Should the Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-p-96T 96T
EUR 715
  • Should the Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-48T 48T
EUR 508
  • Should the Rat Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
DLR-LIF-Ra-96T 96T
EUR 661
  • Should the Rat Leukemia Inhibitory Factor (LIF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-48Tests 48 Tests
EUR 555
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-b-96Tests 96 Tests
EUR 771
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-48Tests 48 Tests
EUR 533
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-c-96Tests 96 Tests
EUR 740
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-48Tests 48 Tests
EUR 367
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Hu-96Tests 96 Tests
EUR 502
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-48Tests 48 Tests
EUR 555
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-p-96Tests 96 Tests
EUR 771
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-48Tests 48 Tests
EUR 511
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RD-LIF-Ra-96Tests 96 Tests
EUR 709
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-48Tests 48 Tests
EUR 580
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-b-96Tests 96 Tests
EUR 807
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-48Tests 48 Tests
EUR 557
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-c-96Tests 96 Tests
EUR 774
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-48Tests 48 Tests
EUR 383
Human Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Hu-96Tests 96 Tests
EUR 525
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-48Tests 48 Tests
EUR 580
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-p-96Tests 96 Tests
EUR 807
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-48Tests 48 Tests
EUR 534
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit
RDR-LIF-Ra-96Tests 96 Tests
EUR 742
anti- LIF antibody
FNab09804 100µg
EUR 548.75
  • Recommended dilution: WB: 1:200-1:1000
  • IHC: 1:50-1:500
  • Immunogen: leukemia inhibitory factor
  • Uniprot ID: P15018
  • Research Area: Signal Transduction, Immunology, Developmental biology, Neuroscience, Stem Cells
Description: Antibody raised against LIF
Anti-LIF Antibody
A01400 100ug/vial
EUR 294
Anti-LIF Antibody
PB9036 100ug/vial
EUR 334
anti- LIF antibody
LSMab09804 100 ug
EUR 386
Anti-LIF Antibody
PA1562 100ug/vial
EUR 294
Anti-LIF antibody
STJ73631 100 µg
EUR 359
Anti-LIF antibody
STJ96472 200 µl
EUR 197
Description: Rabbit polyclonal to LIF.
Anti-LIF antibody
STJ29846 100 µl
EUR 277
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
LIF, Mouse
HY-P7084 50ug
EUR 533
LIF, mouse
MO16102 100 ug
EUR 675
Anti-LIF (aa28-39) antibody
STJ72919 100 µg
EUR 359
Mouse LIF ELISA Kit
EML0014 96Tests
EUR 521
Mouse LIF shRNA Plasmid
20-abx971321
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.
Recombinant Mouse LIF Protein
R01400-1 100ug/vial
EUR 294
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280
rHu LIF
AK9977-0005 5µg Ask for price
rHu LIF
AK9977-0025 25µg Ask for price
rHu LIF
AK9977-0100 100µg Ask for price
rHu LIF
AK9977-1000 1mg Ask for price
LIF, Human
HY-P7049 10ug
EUR 1600
LIF siRNA
20-abx922546
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.
LIF siRNA
20-abx922547
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.
LIF antibody
70R-51295 100 ul
EUR 244
Description: Purified Polyclonal LIF antibody
LIF antibody
70R-6230 50 ug
EUR 467
Description: Rabbit polyclonal LIF antibody
LIF Antibody
36950-100ul 100ul
EUR 252
LIF Antibody
5216-100
EUR 316
LIF Antibody
5216-30T
EUR 146
LIF protein
30R-2771 10 ug
EUR 369
Description: Purified recombinant Human LIF protein
LIF protein
30R-2772 10 ug
EUR 489
Description: Purified recombinant Mouse LIF protein
LIF Antibody
25283-100ul 100ul
EUR 390
LIF antibody
70R-12299 100 ug
EUR 403
Description: Rabbit polyclonal LIF antibody
LIF antibody
70R-14100 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal LIF antibody
LIF Antibody
1-CSB-PA004761
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
LIF Antibody
1-CSB-PA012928ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Antigen Affinity Purified
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA
LIF Antibody
1-CSB-PA198481
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100
LIF antibody
PAab09804 100 ug
EUR 386
LIF, human
MO16101 100 ug
EUR 688
ELISA kit for Mouse LIF
EK5250 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.
Mouse LIF PicoKine ELISA Kit
EK0580 96 wells
EUR 425
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).
Human CellExp? LIF, mouse recombinant
7268-10
EUR 370
Leukemia Inhibitory Factor (LIF), mouse
PR16102 5 ug
EUR 435
LIF, Mouse Recombinant , 1MIU/ml
L4500-001 1ml
EUR 339
LIF, Mouse Recombinant , 1MIU/ml
L4500-005 5x1ml
EUR 1193
LIF, Mouse Recombinant , 10MIU/ml
L4501-001 1ml
EUR 1454
Lif ORF Vector (Mouse) (pORF)
ORF049145 1.0 ug DNA
EUR 506
Lif ORF Vector (Mouse) (pORF)
ORF049146 1.0 ug DNA
EUR 506
LIF ELISA Kit (Mouse) (OKEH04045)
OKEH04045 96 Wells
EUR 544
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL
LIF ELISA Kit (Mouse) (OKBB00760)
OKBB00760 96 Wells
EUR 505
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml
Polyclonal LIF Antibody
APR00279G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LIF . This antibody is tested and proven to work in the following applications:
LIF Conjugated Antibody
C36950 100ul
EUR 397
Polyclonal LIF Antibody
APR06696G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LIF . This antibody is tested and proven to work in the following applications:
LIF Polyclonal Antibody
ES3837-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
LIF Polyclonal Antibody
ES3837-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
LIF Blocking Peptide
20-abx064170
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
LIF Polyclonal Antibody
ABP52838-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Polyclonal Antibody
ABP52838-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Polyclonal Antibody
ABP52838-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human LIF
  • Applications tips:
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
LIF Rabbit pAb
A1288-100ul 100 ul
EUR 308
LIF Rabbit pAb
A1288-200ul 200 ul
EUR 459
LIF Rabbit pAb
A1288-20ul 20 ul
EUR 183
LIF Rabbit pAb
A1288-50ul 50 ul
EUR 223
LIF Blocking Peptide
33R-4712 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of LIF antibody, catalog no. 70R-6230
LIF Polyclonal Antibody
41551-100ul 100ul
EUR 252
LIF Polyclonal Antibody
41551-50ul 50ul
EUR 187
LIF, human recombinant
4923-10
EUR 305
LIF, human recombinant
4923-1000
EUR 5705
LIF, human recombinant
4923-50
EUR 664
LIF, murine recombinant
4924-10
EUR 370
LIF, murine recombinant
4924-1000
EUR 6836

 

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