De uitdagingen van de ontwikkeling van vaccins tegen Betacoronaviruses: Antibody afhankelijke versterking en Sendai Virus als een mogelijke vaccin Vector
Om een effectief en veilig vaccin tegen bètacoronavirussen te ontwerpen, is het noodzakelijk om hun evolutionair conservatieve antigene determinanten te gebruiken die de combinatie van sterke humorale en celgemedieerde immuunresponsen zullen opwekken. Het richten op dergelijke determinanten minimaliseert het risico van antilichaamafhankelijke versterking van virale infectie. Dit fenomeen werd waargenomen in dierproeven met experimentele vaccins tegen SARS-CoV-1 en MERS-CoV die waren ontwikkeld op foundation van geïnactiveerd coronavirus of vectorconstructen die het spike-eiwit (S) van het virion tot expressie brengen.
De substitutie en glycosylering van bepaalde aminozuren in de antigene determinanten van het S-eiwit, evenals de conformationele veranderingen, kunnen tot hetzelfde impact leiden in een nieuw experimenteel vaccin tegen SARS-CoV-2. Het gebruik van meer conservatieve structurele en aanvullende virale eiwitten voor de antigene determinanten van het vaccin zal dit probleem helpen voorkomen. Deze evaluation schetst benaderingen voor het ontwikkelen van vaccins tegen het nieuwe SARS-CoV-2-coronavirus die zijn gebaseerd op niet-pathogene virale vectoren.
Voor een efficiënte preventie van infecties veroorzaakt door respiratoire pathogenen is het vermogen van het vaccin om mucosale immuniteit in de luchtwegen te stimuleren belangrijk. Een dergelijk vaccin kan worden ontwikkeld met behulp van een niet-pathogene Sendai-virusvector, omdat het intranasaal kan worden toegediend en een mucosale immuunrespons kan induceren die de antivirale barrière in de luchtwegen versterkt en een betrouwbare bescherming biedt tegen infectie.
Detectie van mutaties in gyrB met behulp van denaturerende hogedrukvloeistofchromatografie ( DHPLC ) tussen Salmonella enterica serovar Typhi en Paratyphi A.
Fluorochinolonresistentie wordt gemedieerd door mutaties in het chinolonenresistentiebepalende gebied (QRDR) van de topoisomerasegenen. Denaturerende hogedrukvloeistofchromatografie (DHPLC) werd geëvalueerd voor de detectie van klinisch belangrijke mutaties in gyrB bij Salmonella.
Salmonella Typhi en S. Paratyphi A, gekarakteriseerd voor mutatie in QRDR van gyrA, parC en parE, werden onderzocht op mutatie in gyrB door DHPLC en gevalideerd door sequencing.
De DHPLC-analyse was in staat om de testmutant te onderscheiden van isolaten met wildtype gyrB en mutanten te onderscheiden van andere mutanten op foundation van piekprofiel en verschuiving in retentietijd. Er werden drie sequentievarianten gedetecteerd op codon 464 en er werd ook een nieuwe mutatie Ser→Thr gedetecteerd . gyrB-mutatie was geassocieerd met niet-klassieke chinolonresistentie (NALS-CIPDS) in 34 isolaten van alleen S. Typhi en was verschillend van klassieke chinolonresistentie geassocieerd met gyrA-mutaties (NALR-CIPDS).
Opstelling van een protocol voor moleculaire diagnose van β-thalassemie-mutaties in Tunesië met behulp van denaturerende high-performance vloeistofchromatografie ( DHPLC ).
ACHTERGROND
β-thalassemie is een van de meest voorkomende autosomaal recessieve aandoeningen wereldwijd . Het vertoont een grote moleculaire heterogeniteit als gevolg van meer dan 200 oorzakelijke mutaties in het β-globine-gen. In Tunesië vertegenwoordigt β- thalassemie de meest voorkomende monogene hemoglobinestoornis met 2,21% van de dragers.
Efficiënte en betrouwbare screeningsmethoden voor mutaties zijn essentieel om geschikte preventieprogramma’s voor risicoparen op te zetten. Het doel van de huidige studie is om een efficiënte methode te ontwikkelen op foundation van de denaturerende hogedrukvloeistofchromatografie (DHPLC) waarbij het hele β-globinegen (HBB) wordt gescreend op mutaties die ongeveer 90% van het spectrum beslaan.
METHODEN
We hebben de validatie uitgevoerd van een DHPLC-assay voor directe genotypering van 11 bekende β-thalassemie-mutaties in de Tunesische bevolking.
RESULTATEN
De DHPLC-assay werd vastgesteld op foundation van de analyse van 62 gearchiveerde β-thalassemiemonsters die eerder waren gegenotypeerd en vervolgens werden gevalideerd met volledige overeenstemming op 50 exams met blinde gerandomiseerde monsters die eerder waren gegenotypeerd met DNA-sequencing en met 96% consistentie op 40 monsters als een prospectieve studie.
CONCLUSIES
In vergelijking met andere genotyperingstechnieken kan de DHPLC-methode voldoen aan de vereisten van directe genotypering van bekende β-thalassemie-mutaties in Tunesië en kan het worden toegepast als een krachtig hulpmiddel voor de genetische screening van prenatale en postnatale individuen.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds.
Description: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Description: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Description: Mouse Leukemia inhibitory factor（lif）is a secreted protein which belongs to the LIF/OSM family.LIF has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. it has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
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Ontwikkeling van hoge resolutie DNA Melting Analyse voor gelijktijdige detectie van Potato Mop-Prime Virus en Zijn Vector , Spongospora subterranea, in de bodem
In deze studie werd een reeks duplex reverse transcriptie (RT)-PCR-gemedieerde hoge resolutie DNA-smelt (HRM)-analyses voor gelijktijdige detectie van aardappelmop-virus (PMTV) en zijn protistische vector, Spongospora subterranea f.sp. subterranea (Sss), ontwikkeld.
De aantasting van grond door PMTV werd gedetecteerd met behulp van een op tabak gebaseerd lokaassysteem . Totaal RNA geëxtraheerd uit de bodem leidde tot succesvolle RT-PCR-gelelektroforese-detectie van zowel PMTV als Sss. Om efficiëntere detectie te vergemakkelijken, werden nieuw ontworpen primerparen voor PMTV RNA-soorten (dwz RNA-Rep, -CP en -TGB) samen met de bestaande Sss-primers geanalyseerd met behulp van real-time RT-PCR.
De resulterende amplicons vertoonden smeltprofielen die gemakkelijk konden worden onderscheiden. Onder duplex RT-PCR-formaat leidden alle PMTV- en Sss-primercombinaties tot succesvolle detectie van respectieve PMTV-RNA-soorten en Sss in de monsters door DNA- smeltanalyses met hoge resolutie (HRM) . Toen de duplex HRM-assay werd toegepast op bodemmonsters die waren verzameld van zes velden op vier verschillende locaties in New Brunswick, Canada, werd een positieve detectie van PMTV en/of Sss gevonden in 63-100% monsters verzameld uit velden waarin met PMTV geïnfecteerde knollen waren waargenomen.
De monsters van velden waar noch PMTV- noch Sss-geïnfecteerde knollen waren waargenomen, resulteerden daarentegen in een negatieve detectie door de check. Aastabak bioassay voor PMTV en Sss leverde vergelijkbare resultaten op. Tussen 63% -83% en 100% van de grondmonsters die werden verzameld op PMTV-besmette velden leidden tot respectievelijk PMTV- en Sss-infecties in de lokaasplanten; terwijl er geen PMTV- of Sss-geïnfecteerde planten werden verkregen uit grondmonsters die waren verzameld op PMTV/Sss-vrije velden.
Versnelling van de morfogenetische cyclus van de virale vector Aedes aegypti larven voor snellere larvicide bioassays
Elke bioassay om nieuwe chemisch gesynthetiseerde larviciden of fytolarviciden te testen tegen Culicidae en meer schadelijke muggensoorten, zoals Aede s aegypti en Aedes albopictus , die specifiek knokkelkoorts, gele koorts, chikungunya- viruskoorts overbrengen, evenals het Zika-virus of Anopheles gambiae , een vector voor malaria en philariasis, vereist duizenden goed ontwikkelde larven, bij voorkeur in het vierde stadium. De natuurlijke morfogenetische cyclus van Aedes spp., in het veld of in het laboratorium, kan bij kamertemperatuur (bijv. 25°C) tot 19 dagen duren vanaf het eerste permanente contact tussen levensvatbare eieren en wateren het laatste stadium van larvale groei of metamorfose tot vliegende volwassenen. Een versnelde sequentiële vervelling is dus wenselijk voor snellere bioassays van larviciden.
We hebben dit doel bereikt in Aedes aegypti met zeer beperkte strategische en goedkope toevoegingen aan voedsel, zoals kokoswater, melk of caseïne, gistextract en in mindere mate glycerol. Het van nature rijke kokoswater was uitstekend geschikt om snel de populatie van instar IV-larven te bereiken , de meest geavanceerde vóór de verpopping, wat ongeveer een week bespaart voor daaropvolgende larvicide bioassays. Verdunde melk, als een andere voedselbron, zorgde voor een nog snellere uiteindelijke vervelling en volwassenen zijn nuttig voor taxonomische doeleinden van muggen.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.
Description: LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.7 kDa protein containing 180 amino acid residues, including three disulfide bonds.
Description: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Description: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Description: Mouse Leukemia inhibitory factor（lif）is a secreted protein which belongs to the LIF/OSM family.LIF has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. it has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF
