Technologieën voor het bewerken van genen in combinatie met  virale  vectoren

designblast

designblast

Technologieën voor het bewerken van genen in combinatie met  virale  vectoren  voor translationeel onderzoek naar neurodegeneratieve ziekten

Ziekten van het centrale zenuwstelsel (CZS) behoren van oudsher tot de moeilijkst te behandelen met conventionele farmacologische benaderingen . Dit is te wijten aan een samenloop van factoren, waaronder het beperkte regeneratieve vermogen en de algehele complexiteit van de hersenen, problemen die samenhangen met herhaalde toediening van geneesmiddelen en moeilijkheden bij het afleveren van geneesmiddelen door de bloed-hersenbarrière (BBB). Viraal gemedieerde genoverdracht vormt een aantrekkelijk alternatief voor de levering van therapeutische lading aan het zenuwstelsel. Cruciaal is dat het meestal slechts een enkele injectie vereist, of dat nu een genvervangingsstrategie is voor een erfelijke aandoening of de levering van een genoom- of epigenoommodificerend assemble voor de behandeling van ziekten en aandoeningen van het CZS.

Het is dus begrijpelijk dat er aanzienlijke inspanningen zijn geleverd voor de ontwikkeling van verbeterde vectorsystemen voor genoverdracht naar het CZS. Verschillende virale vectoren zijn natuurlijk afgestemd op hun specifieke toepassingen, maar over het algemeen zouden ze verschillende belangrijke eigenschappen moeten delen . De ideale virale vector omvat een hoge verpakkingscapaciteit, efficiënte genoverdracht gecombineerd met robuuste en aanhoudende expressie, gebrek aan oncogeniteit, toxiciteit en pathogeniteit, en schaalbare productie voor klinische toepassingen. In deze evaluate zullen we aandacht besteden aan virale vectoren die zijn afgeleid van humaan immunodeficiëntievirus sort 1 (lentivirale vectoren; LV’s) en adeno-geassocieerd virus (AAV’s).

De grote belangstelling voor deze vectoren voor virale afgiftesystemen is te wijten aan: (i) robuuste afgifte en langdurige expressie; (ii) efficiënte transductie in postmitotische cellen, inclusief de hersenen; (iii) lage immunogeniciteit en toxiciteit; en (iv) compatibiliteit met geavanceerde fabricagetechnieken. Hier zullen we basisaspecten van LV- en AAV-biologie schetsen, met title gericht op benaderingen en technieken die gericht zijn op het verbeteren van de virale veiligheid.

We zullen ook een aanzienlijk deel van deze beoordeling toewijzen aan de ontwikkeling en het gebruik van LV’s en AAV’s voor levering aan het CZS, met een focus op de genoom- en epigenoombewerkingstools op foundation van geclusterde, regelmatig op afstand staande korte palindroomherhalingen/CRISPR-geassocieerd eiwit. (CRISPR/Cas 9) en de ontwikkeling van nieuwe strategieën voor de behandeling van neurodegeneratieve ziekten (NDD’s).

Methoden in moleculaire cardiologie:   analyse van DHPLC- mutatiedetectie.

Er zijn steeds meer mutaties geïdentificeerd in genen die betrokken zijn bij hartaandoeningen, wat heeft geleid tot nieuwe inzichten in de pathofysiologie van erfelijke hartziekten. Als gevolg van deze bevindingen worden technieken die gespecialiseerd zijn in geautomatiseerde high-throughput-analyse geïmplementeerd om het toenemende aantal diagnostische genetische verzoeken aan te kunnen.

Denaturerende hogedrukvloeistofchromatografie (DHPLC) is zo’n nieuwe techniek die voldoet aan de standards van snelheid, gevoeligheid en nauwkeurigheid. Dit nummer concentreert zich op het basisprincipe van de techniek en illustreert hoe genetische veranderingen kunnen worden geïdentificeerd.

DHPLC- technologie voor high-throughput detectie van mutaties in een durumtarwe TILLING-populatie

ACHTERGROND

Durumtarwe (Triticum turgidum L.) is een graangewas dat veel wordt verbouwd in de mediterrane regio’s; de amberkleurige korrel wordt voornamelijk gebruikt voor de productie van pasta, couscous en typische broden. Single nucleotide polymorphism (SNP) detectietechnologieën en high-throughput mutatie-inductie vormen een nieuwe uitdaging in de tarweveredeling om allelische variatie in grote populaties te identificeren.

De TILLING-strategie maakt gebruik van traditionele chemische mutagenese gevolgd door screening op mismatches van enkele basen om nieuwe mutante loci te identificeren. Hoewel TILLING is gecombineerd met verschillende gevoelige pre-screeningmethoden voor SNP-analyse, vertrouwen de meeste op dure apparatuur. Onlangs is een nieuw goedkoop en tijdbesparend DHPLC- protocol gebruikt in de moleculaire menselijke diagnostiek om onbekende mutaties te detecteren.

RESULTATEN

In dit werk hebben we een nieuwe TILLING-populatie van durumtarwe ontwikkeld (cv. Marco Aurelio) met 0,70-0,85% ethylmethaansulfonaat (EMS). Om de efficiëntie van de mutagene behandelingen te onderzoeken, werd een pilotscreening uitgevoerd op 1.140 mutantlijnen die zich richtten op twee doelwitgenen (Lycopeen-epsilon-cyclase, -LCY en Lycopeen-bèta-cyclase , β-LCY) die betrokken zijn bij het carotenoïdemetabolisme in tarwe granen.

We vereenvoudigen de heteroduplexdetectie met twee goedkope methoden: de enzymatische splitsing (CelI)/agarosegeltechniek en de denaturerende hogedrukvloeistofchromatografie (DHPLC) . Met de CelI/agarose-gelbenadering konden we 31 mutaties identificeren, terwijl de DHPLC-procedure in totaal 46 mutaties voor beide genen detecteerde.

Alle gedetecteerde mutaties werden bevestigd door directe sequencing. De geschatte totale mutatiefrequentie voor de pilot-assay volgens de DHPLC- methodologie was 1/77 kb, wat een hoge waarschijnlijkheid vertegenwoordigt om interessante mutaties in de doelgenen te detecteren.

CONCLUSIES

We hebben de toepasbaarheid en efficiëntie aangetoond van een nieuwe strategie voor de detectie van geïnduceerde variabiliteit. We hebben een nieuwe TILLING-populatie van durumtarwe geproduceerd en gekarakteriseerd die nuttig is voor een beter begrip van de belangrijkste genfuncties. De beschikbaarheid van deze software samen met de TILLING- techniek zal de polymorfismen in kandidaatgenen van agronomisch belangrijke eigenschappen in tarwe uitbreiden.

designblast
designblast

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 583

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Rat Tissue Lysate Kidney Medulla Normal

GWB-F5DEBF 0.15 mg Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Total RNA - Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 156

Genomic DNA - Mouse Normal Tissue: Kidney

D1334142 100 ug
EUR 329

Total Protein - Mouse Normal Tissue: Kidney

P1334142 1 mg
EUR 216

Tissue Lysate (Normal) Human Kidney

GWB-FEAC28 0.15 mg Ask for price

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Kidney tumour with normal tissue array

KD2001 each
EUR 474
Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 221

Kidney cancer tissue array with normal tissues

KD2083 each
EUR 474
Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores

Kidney cancer tissue array with normal tissues

KD721 each
EUR 168
Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-100 1 mg
EUR 628.8

Mouse Normal Tissue: Kidney Whole Cell Lysate

MNK-50 50 ug
EUR 196.8

Total RNA - Monkey (Rhesus) Normal Tissue: Kidney

R1534142-50 50 ug
EUR 229

Kidney tumor tissue array with normal tissue as control

KD961 each
EUR 258
Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Total Protein - Human Adult Normal Tissue: Kidney

P1234142 1 mg
EUR 216

Kidney cancer tissue array with normal tissue as control

KD962 each
EUR 258
Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney

R1534142-Cy 50 ug
EUR 229

Adjacent normal kidney tissue and cancer tissue array

BN07011b each
EUR 203
Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011

Membrane Protein - Human Adult Normal Tissue: Kidney

P3234142 0.1 mg
EUR 311

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

FFPE Total RNA - Human Adult Normal Tissue: Kidney

R2234142 1 ug
EUR 744

Kidney cancer tissue array with matched adjacent normal tissue

KD321a each
EUR 198
Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue

Kid-060Sur01 each
EUR 954
Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores

Human Adult Kidney (Normal) Whole tissue lysate

HAL-1305 1 mg
EUR 628.8

Monkey (Cyno.) Normal Kidney Whole tissue lysate

MCL-1190 1 mg
EUR 628.8

Monkey (Rhesus) Normal Kidney Whole tissue lysate

MRL-1301 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 377

Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues

Z7020053 5 slides
EUR 1125.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Kidney

T2234142 5 slides
EUR 204

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Multiple Kidney cancer tissue array with normal tissue control from autopsy

KD483 each
EUR 168
Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 478

Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts

9-80267 1 x 100 ml Ask for price

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney

R1234142-P 50 ug
EUR 443

Rat Normal Kidney tissue (single section per slide) (5 slides/pack)

RaFPT007 5 slides
EUR 150

Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack

TS-R5405 -
EUR 145

High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

KD208 each
EUR 546
Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue

Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor

D1234142 100 ug
EUR 329

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 500

Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes

9-80172 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 424

Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue

KD242 each
EUR 48
Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 636

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 424

Kidney cancer with matched cancer adjacent normal tissue test array

KD241 each
EUR 48
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores

Kidney cancer with matched cancer adjacent normal tissue test array

KD241a each
EUR 66
Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241

cDNA - Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 636

Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80262 1 x 100 ml Ask for price

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 716

Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes

9-80079 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells

9-80266 1 x 100 ml Ask for price

Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80269 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts

9-80077 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts

9-80174 1 x 100 ml Ask for price

Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma

KD951a each
EUR 1434
Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951

Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack

TS-H5013 -
EUR 145

Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack

TS-M5203 -
EUR 145

Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells

9-80078 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells

9-80175 1 x 100 ml Ask for price

cDNA - Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 636

Matched Pair (Normal and Carcinoma) Human Kidney Tissue Array (5 slides/pk)

TAS-1007 1 pk
EUR 343.2

30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array

KD805 each
EUR 258
Description: 30 case of Kidney clear cell carcinoma with 5 of NAT and 5 normal tissue array, 40 cases/80 cores

Human Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80074 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells

9-80170 1 x 100 ml Ask for price

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 595

Paired Cancer and Normal Kidney Paraffin Tissue (FFPE) Section (5 slides/pack)

NCT176 5 slides
EUR 300

Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells

9-80076 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 7: Normal Renal Tubular Epithelial Cells

9-80080 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 4: Normal Renal Artery Endothelial Cells

9-80173 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells

9-80177 1 x 100 ml Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 383

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 424

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-1 1 slide
EUR 761

Frozen Tissue Array - 37 Different Kidney Tumors and 3 Corresponding Normal Controls

T6235142-5 5 slides
EUR 2500

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 424

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 424

Een efficiënte virus -geïnduceerde genuitschakeling (VIGS) voor useful genomics in Brassica by way of een kool leaf curl  virus  (CaLCuV) -gebaseerde  vector

Op CaLCuV gebaseerde VIGS werkt effectief in kool en draagt ​​bij aan efficiënt functioneel genomics-onderzoek in Brassica-gewassen. Virus-geïnduceerde gene silencing (VIGS), een posttranscriptionele gene silencing methode, is een effectieve techniek voor het analyseren van de functies van genen in planten. Tot nu toe waren er echter geen VIGS-vectoren beschikbaar voor Brassica oleracea.

Hier werden tabaksratelvirus (TRV), pTY’s en koolbladkrulvirus (CaLCuV) genuitschakelingsvectoren (PCVA/PCVB) gekozen om het VIGS-systeem in kool te verbeteren met behulp van het fytoeendesaturase (PDS)-gen als een efficiënte visuele indicator van VIGS. We hebben met succes de expressie van PDS tot zwijgen gebracht en hebben fotobleekverschijnselen waargenomen in kool als reactie op pTY’s en CaLCuV, waarbij de laatste eenvoudiger te bedienen en goedkoper is.

De parameters die mogelijk de silencing-efficiëntie van VIGS door CaLCuV in kool beïnvloeden, inclusief de targeting- fragmentstrategie, inoculatiemethode en incubatietemperatuur , werden vervolgens vergeleken. Het geoptimaliseerde op CaLCuV gebaseerde VIGS-systeem omvat het volgende: een insertiesequentie van ongeveer 500 bp, een Agrobacterium OD 600  van 1,0, gebruik van de vacuümosmosemethode toegepast in het kiemstadium en een incubatietemperatuur van 22 °C.

Met behulp van deze parameters hebben we een stabiel dempingsrendement van 65% bereikt. Om de effectiviteit van het systeem verder te testen, selecteerden we het Mg-chelatase H-subeenheid (ChlH) -gen in kool en schakelden de expressie ervan uit, en we observeerden gele bladeren , zoals verwacht. We hebben het op CaLCuV gebaseerde VIGS-systeem met succes toegepast op twee andere representatieve Brassica-gewassen, B. rapa en B. nigra, en hebben zo het toepassingsgebied van dit systeem uitgebreid. Ons hier beschreven VIGS-systeem zal bijdragen aan efficiënt functioneel genomics-onderzoek in Brassica-gewassoorten.

Oculaire Gentherapie met adeno-geassocieerde  virus  vectoren : Actueel Outlook voor patiënten en onderzoekers

In dit “Perspectief” bespreken we oculaire gentherapie – het perspectief van de patiënt, de verschillende strategieën van genvervanging en genbewerking, de plaats van adeno-geassocieerde virusvectoren, routes van levering aan het oog en de resterende vraag – “waarom werkt immuniteit werkzaamheid blijven beperken?” Door de gecoördineerde inspanningen van patiënten, onderzoekers, subsidieverlenende instanties en de industrie, en na vele jaren van preklinische research, biochemische, cellulaire en diermodellen, zien we klinische proeven ontstaan ​​voor veel voorheen onbehandelbare erfelijke oculaire aandoeningen.

De weg naar therapieën werd geleid door de succesvolle behandeling van de RPE65 vorm van Leber congenitale amaurose met LUXTURNA TM . In sommige gevallen blijven immuunreacties op de vectoren optreden, waardoor de werkzaamheid wordt beperkt. De onderliggende ontstekingsmechanismen vereisen verder onderzoek en er moeten nieuwe vectoren worden ontworpen die de triggers van immuniteit beperken .

Onderzoekers die oculaire gentherapieën bestuderen en clinici die patiënten inschrijven voor klinische onderzoeken, moeten de huidige beperkingen van deze therapieën erkennen om de verwachtingen goed te managen en teleurstelling te voorkomen, maar we zijn van mening dat gentherapieën goed op weg zijn naar succesvol, wijdverbreid gebruik voor de behandeling van erfelijke oculaire aandoeningen.

Mouse Podoplanin ELISA kit

E03P0082-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Podoplanin ELISA kit

E03P0082-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Podoplanin ELISA kit

E03P0082-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Podoplanin ELISA Kit

IMSPDPNKT each
EUR 752
Description: Mouse Podoplanin ELISA Kit

anti- Podoplanin antibody

FNab06598 100µg
EUR 702
Description: Antibody raised against Podoplanin

anti- Podoplanin antibody

FNab06599 100µg
EUR 702
Description: Antibody raised against Podoplanin

Mouse Monoclonal anti-Rat Podoplanin Antibody

xAP-0897 100ug
EUR 280

Mouse Podoplanin (PDPN) Protein

20-abx650139
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Podoplanin (PDPN) Protein

abx694266-10nmol 10 nmol
EUR 700

Mouse Podoplanin (PDPN) Protein

abx694266-5nmol 5 nmol
EUR 462.5

Mouse Podoplanin (PDPN) Protein

abx650139-100g 100 µg
EUR 537.5

Mouse Podoplanin (PDPN) Protein

abx650139-10g 10 µg
EUR 212.5

Mouse Podoplanin (PDPN) Protein

abx650139-50g 50 µg
EUR 375

Mouse Monoclonal Podoplanin Antibody

TA327727 1 ml Ask for price

Mouse Podoplanin (PDPN) CLIA Kit

20-abx493858
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Podoplanin (PDPN) ELISA Kit

DLR-PDPN-Mu 96T
EUR 475
Description: tissue homogenates, cell lysates or other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

DLR-PDPN-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

DLR-PDPN-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

EK8596 96Т
EUR 799

Mouse Podoplanin, Pdpn ELISA KIT

ELI-06723m 96 Tests
EUR 1038

Mouse Podoplanin (PDPN) ELISA Kit

EKN47838-48T 48T
EUR 389.62

Mouse Podoplanin (PDPN) ELISA Kit

EKN47838-5x96T 5x96T
EUR 2643.85

Mouse Podoplanin (PDPN) ELISA Kit

EKN47838-96T 96T
EUR 556.6

Mouse Podoplanin (PDPN) ELISA Kit

EKU06727-48T 48T
EUR 571.55

Mouse Podoplanin (PDPN) ELISA Kit

EKU06727-5x96T 5x96T
EUR 3878.38

Mouse Podoplanin (PDPN) ELISA Kit

EKU06727-96T 96T
EUR 816.5

Mouse Podoplanin (PDPN) ELISA Kit

DL-PDPN-Mu 96T
EUR 453
Description: tissue homogenates, cell lysates or other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

abx571749-96tests 96 tests
EUR 943.2

Mouse Podoplanin (PDPN) ELISA Kit

AE28131MO-48Tests 48 Tests
EUR 325
Description: Mouse (Mus musculus)

Mouse Podoplanin (PDPN) ELISA Kit

AE28131MO-96Tests 96 Tests
EUR 610
Description: Mouse (Mus musculus)

Mouse Podoplanin (PDPN) ELISA Kit

20-abx154564
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-48T 48T
EUR 464.3
Description: tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-48Tests 48 Tests
EUR 639.6

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-96T 96T
EUR 663.3
Description: tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-96Tests 96 Tests
EUR 888

Mouse Podoplanin (PDPN) ELISA Kit

RK13459 96T
EUR 280

Mouse Podoplanin(PDPN)ELISA kit

QY-E21215 96T
EUR 433.2

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

4-SEC719Mu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-48T 48T
EUR 487.52
Description: tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-48Tests 48 Tests
EUR 668.4

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-96T 96T
EUR 696.47
Description: tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-96Tests 96 Tests
EUR 928.8

Mouse Podoplanin (PDPN)ELISA kit

YLA0596MO-48T 48T Ask for price

Mouse Podoplanin (PDPN)ELISA kit

YLA0596MO-96T 96T Ask for price

Mouse PDPN(Podoplanin) ELISA Kit

ELK7080-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDPN in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse PDPN(Podoplanin) ELISA Kit

ELK7080-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDPN in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse PDPN (Podoplanin) ELISA Kit

EKE61859-5x96T 5x96T
EUR 3124.08

Mouse PDPN (Podoplanin) ELISA Kit

EKE61859-96T 96T
EUR 657.7

Mouse PDPN(Podoplanin) ELISA Kit

RE2052M-48wells 48 wells
EUR 116.55

Mouse PDPN(Podoplanin) ELISA Kit

RE2052M-96wells 96 wells
EUR 161.55

Anti-Rat Podoplanin Antibody

104-M40 100 µg
EUR 189
Description: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting.

Anti- Podoplanin Human Antibody

GWB-F131C7 0.2 mg Ask for price

Podoplanin

E8ER1915-23 100ul
EUR 275
Description: Available in various conjugation types.

Podoplanin

E8ET1703-61 100ul
EUR 275
Description: Available in various conjugation types.

Podoplanin

AP79286 1mg
EUR 2640

Podoplanin

AP86601 1mg
EUR 2640

Podoplanin

AP87092 1mg
EUR 2640

Podoplanin

AP87543 1mg
EUR 2640

Podoplanin

MO47016 100 ul
EUR 418.8

Rabbit Anti-Human Podoplanin

102-PA40 100ug
EUR 240

Recombinant Mouse Podoplanin (C-Fc)

CU73-10ug 10ug
EUR 157.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-500ug 500ug
EUR 1399.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-50ug 50ug
EUR 327.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Mouse Podoplanin, PDPN GENLISA ELISA

KLM1437 1 x 96 wells
EUR 341

Anti-Human Podoplanin Antibody

102-PA40AG 50 µg
EUR 147
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

102-PA40S 100 µg
EUR 126
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

101-M40-FITC 50 µg Ask for price
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

101-M40-PE 50 µg Ask for price
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

101-M40S-FITC 25 µg Ask for price
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

101-M40S-PE 25 µg Ask for price
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Anti-Human Podoplanin Antibody

101-M41 100 µg
EUR 199.5
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Hamster Monoclonal anti-mouse Podoplanin

mAP-0062 100ug
EUR 250

Podoplanin (PDPN) Polyclonal Antibody (Mouse)

4-PAC719Mu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN)

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse PDPN (Podoplanin)

E-EL-M2409 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDPN (Podoplanin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse PDPN (Podoplanin)

ELK7080 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Podoplanin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Podoplanin antibody

10R-7662 100 ug
EUR 754.8
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-7663 100 ug
EUR 754.8
Description: Hamster monoclonal Podoplanin antibody

Podoplanin antibody

10R-7664 100 ug
EUR 754.8
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-P133a 100 ug
EUR 438
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-P155a 100 ug
EUR 468
Description: Hamster monoclonal Podoplanin antibody

Podoplanin Antibody

E38PA2500 100ul
EUR 225
Description: Available in various conjugation types.

Podoplanin Antibody

DF12456 200ul
EUR 420

Podoplanin Antibody

DF12456-100ul 100ul
EUR 280

Podoplanin Antibody

DF12456-200ul 200ul
EUR 350

Podoplanin Antibody

49474 100ul
EUR 499

Podoplanin Antibody

49474-100ul 100ul
EUR 399.6

Podoplanin Antibody

49474-50ul 50ul
EUR 286.8

Podoplanin antibody

70R-PR007 100 ug
EUR 524
Description: Affinity purified Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-13952 100 ug
EUR 484
Description: Affinity purified Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-11540 100 ug
EUR 632.4
Description: Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-6816 50 ug
EUR 467
Description: Rabbit polyclonal Podoplanin antibody raised against the N terminal of PDPN

Podoplanin antibody

70R-6817 50 ug
EUR 467
Description: Rabbit polyclonal Podoplanin antibody raised against the middle region of PDPN

Podoplanin antibody

70R-50819 100 ul
EUR 242
Description: Purified Polyclonal Podoplanin antibody

Podoplanin Antibody

GWB-9E8999 1 ml Ask for price

Podoplanin Antibody

GWB-61B370 0.1 mg Ask for price

Podoplanin Antibody

R20464-0.1ML 100ul
EUR 409

Podoplanin Antibody

R33852-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: PDPN; AGGRUS; GP36; GP40; Gp38; HT1A-1; OTS8; PA2.26

Podoplanin Antibody

P1051-01m 0.1m
EUR 250.8

Podoplanin Antibody

P1051-1ml 1ml
EUR 1129.2

Anti-D2-40 Podoplanin antibody

STJ16100360 1 mL
EUR 1482

Anti-D2-40/Podoplanin antibody

STJ190048 200 µl
EUR 236.4
Description: Unconjugated Mouse monoclonal to D2-40/Podoplanin (7D1)

Podoplanin (PDPN) Polyclonal Antibody (Mouse), PE

4-PAC719Mu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with PE.

Pdpn (untagged) - Mouse podoplanin (Pdpn), (10ug)

MC201786 10 µg Ask for price

Podoplanin (PDPN) Polyclonal Antibody (Mouse), APC

4-PAC719Mu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with APC.

Podoplanin (PDPN) Polyclonal Antibody (Mouse), Cy3

4-PAC719Mu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with Cy3.

Podoplanin (PDPN) Polyclonal Antibody (Mouse), HRP

4-PAC719Mu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with HRP.

Podoplanin Mouse Monoclonal Antibody (IHC Gold)

E44H20290 250ul
EUR 655
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Rat Monoclonal anti-Mouse Podoplanin Antibody

xAP-0812 100ug
EUR 280

Podoplanin (PDPN) Polyclonal Antibody (Mouse), FITC

4-PAC719Mu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with FITC.

Mouse Anti-Human Podoplanin monoclonal antibody, clone JID761

CABT-L2919-100uL500uL 100 uL, 500 uL
EUR 602.4

Mouse Anti-Human Podoplanin monoclonal antibody, clone JID761

CABT-L2919 100 µl, 500 µl Ask for price
Description: Mouse

Mouse Podoplanin, soluble Recombinant Protein

S01-M46 5 µg
EUR 63
Description: Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Podoplanin (PDPN) Antibody

abx433110-200ul 200 ul
EUR 460.8

Podoplanin (PDPN) Antibody

20-abx339428
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  • 100 ul
  • 50 ul

Podoplanin (PDPN) Antibody

20-abx129101
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Podoplanin (PDPN) Antibody

20-abx131102
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Podoplanin (PDPN) Antibody

20-abx211099
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  • 100 ul
  • 50 ul

Podoplanin (PDPN) Antibody

20-abx174125
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  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx174126
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  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx178065
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  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx178066
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  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx178067
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  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx123399
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  • 100 ul
  • 200 ul
  • 50 ul

Podoplanin (PDPN) Antibody

abx236598-100ug 100 ug
EUR 661.2

Podoplanin (PDPN) Antibody

abx236599-100ug 100 ug
EUR 661.2

Podoplanin (PDPN) Antibody

20-abx137407
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  • 100 ug
  • 20 ug
  • 5 ug

Podoplanin (PDPN) Antibody

20-abx114559
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  • 150 ul
  • 50 ul

Podoplanin (PDPN) Antibody

20-abx005408
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Podoplanin (PDPN) Antibody

20-abx008317
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  • 100 ul
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  • 30 ul

Pdpn Antibody /Podoplanin

RQ6390 100 ug
EUR 356.15
Description: Podoplanin (PDPN) is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the T1A gene to chromosome 1. It has got 162 amino acid protein which is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. Northern blot analysis detected a 1.2-kb T1A2 transcript only in lung. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury.

Podoplanin Antibody gp36

R30481 100 ug
EUR 356.15
Description: Podoplanin is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the gene to chromosome 1. It is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury.

Podoplanin Antibody / gp36

R30760 100 ug
EUR 356.15
Description: PDPN (Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.

Podoplanin (PDPN) Antibody

abx005408-100l 100 µl
EUR 175

Podoplanin Antibody / gp36

V8695-100UG 100 ug
EUR 349.3
Description: It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin Antibody / gp36

V8695-20UG 20 ug
EUR 153.3
Description: It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin Antibody / gp36

V8695SAF-100UG 100 ug
EUR 349.3
Description: It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin Antibody / gp36

V3404-100UG 100 ug
EUR 424.15
Description: Podoplanin (PDPN) is a muco-protein which localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin Antibody / gp36

V3404-20UG 20 ug
EUR 186.15
Description: Podoplanin (PDPN) is a muco-protein which localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin Antibody / gp36

V3404SAF-100UG 100 ug
EUR 424.15
Description: Podoplanin (PDPN) is a muco-protein which localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Podoplanin (PDPN) Antibody

abx236598-100g 100 µg
EUR 350

Podoplanin (PDPN) Antibody

abx236599-100g 100 µg
EUR 350

Podoplanin (PDPN) Antibody

abx114559-100l 100 µl
EUR 612.5

Podoplanin (PDPN) Antibody

abx129101-100l 100 µl
EUR 275

Podoplanin (PDPN) Antibody

abx129101-1ml 1 ml
EUR 750

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