Technologieën voor het bewerken van genen in combinatie met  virale  vectoren

designblast

designblast

Technologieën voor het bewerken van genen in combinatie met  virale  vectoren  voor translationeel onderzoek naar neurodegeneratieve ziekten

Ziekten van het centrale zenuwstelsel (CZS) behoren van oudsher tot de moeilijkst te behandelen met conventionele farmacologische benaderingen . Dit is te wijten aan een samenloop van factoren, waaronder het beperkte regeneratieve vermogen en de algehele complexiteit van de hersenen, problemen die samenhangen met herhaalde toediening van geneesmiddelen en moeilijkheden bij het afleveren van geneesmiddelen door de bloed-hersenbarrière (BBB). Viraal gemedieerde genoverdracht vormt een aantrekkelijk alternatief voor de levering van therapeutische lading aan het zenuwstelsel. Cruciaal is dat het meestal slechts een enkele injectie vereist, of dat nu een genvervangingsstrategie is voor een erfelijke aandoening of de levering van een genoom- of epigenoommodificerend assemble voor de behandeling van ziekten en aandoeningen van het CZS.

Het is dus begrijpelijk dat er aanzienlijke inspanningen zijn geleverd voor de ontwikkeling van verbeterde vectorsystemen voor genoverdracht naar het CZS. Verschillende virale vectoren zijn natuurlijk afgestemd op hun specifieke toepassingen, maar over het algemeen zouden ze verschillende belangrijke eigenschappen moeten delen . De ideale virale vector omvat een hoge verpakkingscapaciteit, efficiënte genoverdracht gecombineerd met robuuste en aanhoudende expressie, gebrek aan oncogeniteit, toxiciteit en pathogeniteit, en schaalbare productie voor klinische toepassingen. In deze evaluate zullen we aandacht besteden aan virale vectoren die zijn afgeleid van humaan immunodeficiëntievirus sort 1 (lentivirale vectoren; LV’s) en adeno-geassocieerd virus (AAV’s).

De grote belangstelling voor deze vectoren voor virale afgiftesystemen is te wijten aan: (i) robuuste afgifte en langdurige expressie; (ii) efficiënte transductie in postmitotische cellen, inclusief de hersenen; (iii) lage immunogeniciteit en toxiciteit; en (iv) compatibiliteit met geavanceerde fabricagetechnieken. Hier zullen we basisaspecten van LV- en AAV-biologie schetsen, met title gericht op benaderingen en technieken die gericht zijn op het verbeteren van de virale veiligheid.

We zullen ook een aanzienlijk deel van deze beoordeling toewijzen aan de ontwikkeling en het gebruik van LV’s en AAV’s voor levering aan het CZS, met een focus op de genoom- en epigenoombewerkingstools op foundation van geclusterde, regelmatig op afstand staande korte palindroomherhalingen/CRISPR-geassocieerd eiwit. (CRISPR/Cas 9) en de ontwikkeling van nieuwe strategieën voor de behandeling van neurodegeneratieve ziekten (NDD’s).

Methoden in moleculaire cardiologie:   analyse van DHPLC- mutatiedetectie.

Er zijn steeds meer mutaties geïdentificeerd in genen die betrokken zijn bij hartaandoeningen, wat heeft geleid tot nieuwe inzichten in de pathofysiologie van erfelijke hartziekten. Als gevolg van deze bevindingen worden technieken die gespecialiseerd zijn in geautomatiseerde high-throughput-analyse geïmplementeerd om het toenemende aantal diagnostische genetische verzoeken aan te kunnen.

Denaturerende hogedrukvloeistofchromatografie (DHPLC) is zo’n nieuwe techniek die voldoet aan de standards van snelheid, gevoeligheid en nauwkeurigheid. Dit nummer concentreert zich op het basisprincipe van de techniek en illustreert hoe genetische veranderingen kunnen worden geïdentificeerd.

DHPLC- technologie voor high-throughput detectie van mutaties in een durumtarwe TILLING-populatie

ACHTERGROND

Durumtarwe (Triticum turgidum L.) is een graangewas dat veel wordt verbouwd in de mediterrane regio’s; de amberkleurige korrel wordt voornamelijk gebruikt voor de productie van pasta, couscous en typische broden. Single nucleotide polymorphism (SNP) detectietechnologieën en high-throughput mutatie-inductie vormen een nieuwe uitdaging in de tarweveredeling om allelische variatie in grote populaties te identificeren.

De TILLING-strategie maakt gebruik van traditionele chemische mutagenese gevolgd door screening op mismatches van enkele basen om nieuwe mutante loci te identificeren. Hoewel TILLING is gecombineerd met verschillende gevoelige pre-screeningmethoden voor SNP-analyse, vertrouwen de meeste op dure apparatuur. Onlangs is een nieuw goedkoop en tijdbesparend DHPLC- protocol gebruikt in de moleculaire menselijke diagnostiek om onbekende mutaties te detecteren.

RESULTATEN

In dit werk hebben we een nieuwe TILLING-populatie van durumtarwe ontwikkeld (cv. Marco Aurelio) met 0,70-0,85% ethylmethaansulfonaat (EMS). Om de efficiëntie van de mutagene behandelingen te onderzoeken, werd een pilotscreening uitgevoerd op 1.140 mutantlijnen die zich richtten op twee doelwitgenen (Lycopeen-epsilon-cyclase, -LCY en Lycopeen-bèta-cyclase , β-LCY) die betrokken zijn bij het carotenoïdemetabolisme in tarwe granen.

We vereenvoudigen de heteroduplexdetectie met twee goedkope methoden: de enzymatische splitsing (CelI)/agarosegeltechniek en de denaturerende hogedrukvloeistofchromatografie (DHPLC) . Met de CelI/agarose-gelbenadering konden we 31 mutaties identificeren, terwijl de DHPLC-procedure in totaal 46 mutaties voor beide genen detecteerde.

Alle gedetecteerde mutaties werden bevestigd door directe sequencing. De geschatte totale mutatiefrequentie voor de pilot-assay volgens de DHPLC- methodologie was 1/77 kb, wat een hoge waarschijnlijkheid vertegenwoordigt om interessante mutaties in de doelgenen te detecteren.

CONCLUSIES

We hebben de toepasbaarheid en efficiëntie aangetoond van een nieuwe strategie voor de detectie van geïnduceerde variabiliteit. We hebben een nieuwe TILLING-populatie van durumtarwe geproduceerd en gekarakteriseerd die nuttig is voor een beter begrip van de belangrijkste genfuncties. De beschikbaarheid van deze software samen met de TILLING- techniek zal de polymorfismen in kandidaatgenen van agronomisch belangrijke eigenschappen in tarwe uitbreiden.

designblast
designblast

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 217.25
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

cDNA from Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Kidney

C1236142Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Matching Pair - cDNA from Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 499
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney

D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Kidney

R1434142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Monkey (Cynomolgus) cDNA Normal Tissue: Liver

MC34-149 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Pancreas

MC34-188 10 rxn
EUR 415

Monkey (Cynomolgus) cDNA Normal Tissue: Spleen

MC34-246 10 rxn
EUR 415

cDNA from Mouse Normal Tissue: Adipose

C1334003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Bladder

C1334010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Colon

C1334090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Heart

C1334122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Liver

C1334149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Placenta

C1334200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Rectum

C1334206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Spleen

C1334246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Stomach

C1334248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Testis

C1334260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Esophagus

C1734106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Pancreas

C1734188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 939
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Skeletal Muscle

C1334171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adipose

C1534003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adipose

C1534003-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Appendix

C1534006 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Appendix

C1534006-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Een efficiënte virus -geïnduceerde genuitschakeling (VIGS) voor useful genomics in Brassica by way of een kool leaf curl  virus  (CaLCuV) -gebaseerde  vector

Op CaLCuV gebaseerde VIGS werkt effectief in kool en draagt ​​bij aan efficiënt functioneel genomics-onderzoek in Brassica-gewassen. Virus-geïnduceerde gene silencing (VIGS), een posttranscriptionele gene silencing methode, is een effectieve techniek voor het analyseren van de functies van genen in planten. Tot nu toe waren er echter geen VIGS-vectoren beschikbaar voor Brassica oleracea.

Hier werden tabaksratelvirus (TRV), pTY’s en koolbladkrulvirus (CaLCuV) genuitschakelingsvectoren (PCVA/PCVB) gekozen om het VIGS-systeem in kool te verbeteren met behulp van het fytoeendesaturase (PDS)-gen als een efficiënte visuele indicator van VIGS. We hebben met succes de expressie van PDS tot zwijgen gebracht en hebben fotobleekverschijnselen waargenomen in kool als reactie op pTY’s en CaLCuV, waarbij de laatste eenvoudiger te bedienen en goedkoper is.

De parameters die mogelijk de silencing-efficiëntie van VIGS door CaLCuV in kool beïnvloeden, inclusief de targeting- fragmentstrategie, inoculatiemethode en incubatietemperatuur , werden vervolgens vergeleken. Het geoptimaliseerde op CaLCuV gebaseerde VIGS-systeem omvat het volgende: een insertiesequentie van ongeveer 500 bp, een Agrobacterium OD 600  van 1,0, gebruik van de vacuümosmosemethode toegepast in het kiemstadium en een incubatietemperatuur van 22 °C.

Met behulp van deze parameters hebben we een stabiel dempingsrendement van 65% bereikt. Om de effectiviteit van het systeem verder te testen, selecteerden we het Mg-chelatase H-subeenheid (ChlH) -gen in kool en schakelden de expressie ervan uit, en we observeerden gele bladeren , zoals verwacht. We hebben het op CaLCuV gebaseerde VIGS-systeem met succes toegepast op twee andere representatieve Brassica-gewassen, B. rapa en B. nigra, en hebben zo het toepassingsgebied van dit systeem uitgebreid. Ons hier beschreven VIGS-systeem zal bijdragen aan efficiënt functioneel genomics-onderzoek in Brassica-gewassoorten.

Oculaire Gentherapie met adeno-geassocieerde  virus  vectoren : Actueel Outlook voor patiënten en onderzoekers

In dit “Perspectief” bespreken we oculaire gentherapie – het perspectief van de patiënt, de verschillende strategieën van genvervanging en genbewerking, de plaats van adeno-geassocieerde virusvectoren, routes van levering aan het oog en de resterende vraag – “waarom werkt immuniteit werkzaamheid blijven beperken?” Door de gecoördineerde inspanningen van patiënten, onderzoekers, subsidieverlenende instanties en de industrie, en na vele jaren van preklinische research, biochemische, cellulaire en diermodellen, zien we klinische proeven ontstaan ​​voor veel voorheen onbehandelbare erfelijke oculaire aandoeningen.

De weg naar therapieën werd geleid door de succesvolle behandeling van de RPE65 vorm van Leber congenitale amaurose met LUXTURNA TM . In sommige gevallen blijven immuunreacties op de vectoren optreden, waardoor de werkzaamheid wordt beperkt. De onderliggende ontstekingsmechanismen vereisen verder onderzoek en er moeten nieuwe vectoren worden ontworpen die de triggers van immuniteit beperken .

Onderzoekers die oculaire gentherapieën bestuderen en clinici die patiënten inschrijven voor klinische onderzoeken, moeten de huidige beperkingen van deze therapieën erkennen om de verwachtingen goed te managen en teleurstelling te voorkomen, maar we zijn van mening dat gentherapieën goed op weg zijn naar succesvol, wijdverbreid gebruik voor de behandeling van erfelijke oculaire aandoeningen.

Podoplanin

MO47016 100 ul
EUR 349

Anti-Podoplanin Rabbit Monoclonal Antibody

M01124-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Podoplanin Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-Podoplanin Rabbit Monoclonal Antibody

M01124-2 100ug/vial
EUR 397
Description: Rabbit Monoclonal Podoplanin Antibody. Validated in WB and tested in Human.

Anti-Podoplanin/gp36/PDPN Antibody

PA1411 100ug/vial
EUR 294

Anti-Podoplanin/gp36/PDPN Antibody

PA1674 100ug/vial
EUR 294

Anti-Podoplanin/gp36/PDPN Antibody

PA1675 100ug/vial
EUR 294

Anti-Podoplanin/gp36/PDPN Antibody

A01124 100ug/vial
EUR 294

Anti-D2-40 Podoplanin antibody

STJ16100360 1 mL
EUR 1235

Anti-D2-40/Podoplanin antibody

STJ190048 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to D2-40/Podoplanin (7D1)

Mouse Podoplanin (PDPN) Protein

20-abx650139
  • EUR 648.00
  • EUR 272.00
  • EUR 1943.00
  • EUR 759.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Podoplanin ELISA kit

E03P0082-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Podoplanin ELISA kit

E03P0082-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Podoplanin ELISA kit

E03P0082-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Anti-Human Podoplanin monoclonal antibody, clone JID761

CABT-L2919-100uL500uL 100 uL, 500 uL
EUR 502

Podoplanin Antibody

P1051-01m 0.1m
EUR 209

Podoplanin Antibody

P1051-1ml 1ml
EUR 941

Podoplanin Antibody

49474-100ul 100ul
EUR 333

Podoplanin Antibody

49474-50ul 50ul
EUR 239

Podoplanin Antibody

DF12456 200ul
EUR 304
Description: Podoplanin antibody detects endogenous levels of Podoplanin.

Podoplanin antibody

10R-7662 100 ug
EUR 629
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-7663 100 ug
EUR 629
Description: Hamster monoclonal Podoplanin antibody

Podoplanin antibody

10R-7664 100 ug
EUR 629
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-P133a 100 ug
EUR 507
Description: Mouse monoclonal Podoplanin antibody

Podoplanin antibody

10R-P155a 100 ug
EUR 543
Description: Hamster monoclonal Podoplanin antibody

Podoplanin antibody

70R-6816 50 ug
EUR 467
Description: Rabbit polyclonal Podoplanin antibody raised against the N terminal of PDPN

Podoplanin antibody

70R-6817 50 ug
EUR 467
Description: Rabbit polyclonal Podoplanin antibody raised against the middle region of PDPN

Podoplanin antibody

70R-PR007 100 ug
EUR 422
Description: Affinity purified Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-11540 100 ug
EUR 527
Description: Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-13952 100 ug
EUR 305
Description: Affinity purified Rabbit polyclonal Podoplanin antibody

Podoplanin antibody

70R-50819 100 ul
EUR 244
Description: Purified Polyclonal Podoplanin antibody

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Mouse Podoplanin (PDPN) ELISA Kit

20-abx154564
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Podoplanin (PDPN) ELISA Kit

abx571749-96tests 96 tests
EUR 786

Mouse Podoplanin (PDPN) CLIA Kit

20-abx493858
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-48Tests 48 Tests
EUR 557

Mouse Podoplanin (PDPN) ELISA Kit

RDR-PDPN-Mu-96Tests 96 Tests
EUR 774

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-48Tests 48 Tests
EUR 533

Mouse Podoplanin (PDPN) ELISA Kit

RD-PDPN-Mu-96Tests 96 Tests
EUR 740

Mouse Podoplanin (PDPN) ELISA Kit

DLR-PDPN-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

DLR-PDPN-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.

Podoplanin (PDPN) Polyclonal Antibody (Mouse)

4-PAC719Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN)

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

SEC719Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.

Mouse Podoplanin (PDPN) ELISA Kit

4-SEC719Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Podoplanin(PDPN)ELISA kit

QY-E21215 96T
EUR 361

Mouse Podoplanin, Pdpn ELISA KIT

ELI-06723m 96 Tests
EUR 865

Recombinant Mouse Podoplanin (C-Fc)

CU73-10ug 10ug
EUR 131
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-1mg 1mg
EUR 1674
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-500ug 500ug
EUR 1166
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Podoplanin (C-Fc)

CU73-50ug 50ug
EUR 273
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Podoplanin (PDPN) Antibody

20-abx131102
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Podoplanin (PDPN) Antibody

20-abx129101
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Podoplanin (PDPN) Antibody

20-abx137407
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Podoplanin (PDPN) Antibody

20-abx114559
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Podoplanin (PDPN) Antibody

20-abx123399
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul

Podoplanin Blocking Peptide

20-abx063694
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Podoplanin (PDPN) Antibody

20-abx008317
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Podoplanin (PDPN) Antibody

20-abx005408
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Recombinant Human Podoplanin

7-05839 5µg Ask for price

Recombinant Human Podoplanin

7-05840 25µg Ask for price

Recombinant Human Podoplanin

7-05841 1mg Ask for price

Podoplanin (PDPN) Antibody

abx433110-200ul 200 ul
EUR 384

Podoplanin (PDPN) Protein

20-abx262003
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

Podoplanin (PDPN) Antibody

20-abx339428
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Podoplanin (PDPN) Antibody

abx236598-100ug 100 ug
EUR 551

Podoplanin (PDPN) Antibody

abx236599-100ug 100 ug
EUR 551

Podoplanin (PDPN) Antibody

20-abx178065
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx178066
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx178067
  • EUR 1414.00
  • EUR 662.00
  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx174125
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx174126
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Podoplanin (PDPN) Antibody

20-abx211099
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Podoplanin Conjugated Antibody

C49474 100ul
EUR 397

Recombinant Podoplanin (PDPN)

4-RPC719Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Podoplanin expressed in: E.coli

Recombinant Podoplanin (PDPN)

4-RPC719Mu01
  • EUR 458.40
  • EUR 226.00
  • EUR 1444.00
  • EUR 548.00
  • EUR 996.00
  • EUR 370.00
  • EUR 3460.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Podoplanin expressed in: E.coli

Podoplanin Blocking Peptide

DF12456-BP 1mg
EUR 195

Podoplanin Blocking Peptide

33R-9441 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PDPN antibody, catalog no. 70R-6817

Podoplanin Blocking Peptide

33R-2420 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PDPN antibody, catalog no. 70R-6816

Podoplanin Recombinant Protein

91-478 0.05 mg
EUR 448.25
Description: Podoplanin is a type-1 transmembrane protein that belongs to Podoplanin family. PDPN expressed in various specialized cell types throughout the body. It highly expressed in placenta, lung, skeletal muscle and brain, weakly expressed in brain, kidney and liver. In placenta, PDPN expressed on the apical plasma membrane of endothelium, in lung, expressed in alveolar epithelium. PDPN physiological function is related to its mucin-type character. PDPN may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, and major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It requires for normal lung cell proliferation and alveolus formation at birth and Induces platelet aggregation. Nevertheless, it doesn’t have any effect on amino acid transport and the aquaporin-type water channels.

Podoplanin Recombinant Protein

91-788 0.05 mg
EUR 490.25
Description: Podoplanin is a type-1 transmembrane protein that belongs to Podoplanin family. PDPN expressed in various specialized cell types throughout the body. It highly expressed in placenta, lung, skeletal muscle and brain, weakly expressed in brain, kidney and liver. In placenta, PDPN expressed on the apical plasma membrane of endothelium, in lung, expressed in alveolar epithelium. PDPN physiological function is related to its mucin-type character. PDPN may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, and major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It requires for normal lung cell proliferation and alveolus formation at birth and Induces platelet aggregation. Nevertheless, it doesn’t have any effect on amino acid transport and the aquaporin-type water channels.

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-1mg 1 mg
EUR 897

Syrian Hamster Anti-Mouse Podoplanin (gp38) Monoclonal antibody, clone 8.1.1

CABT-L4294-5mg 5 mg
EUR 2405

ELISA kit for Mouse PDPN (Podoplanin)

E-EL-M2409 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDPN (Podoplanin) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Podoplanin (PDPN)

KTE70775-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Mouse Podoplanin (PDPN) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse PDPN (Podoplanin)

ELK7080 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Podoplanin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Podoplanin (PDPN) Polyclonal Antibody (Mouse), APC

4-PAC719Mu01-APC
  • EUR 351.00
  • EUR 3365.00
  • EUR 935.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Podoplanin (PDPN). This antibody is labeled with APC.

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